具有二肽基肽酶 IV 抑制活性的鱼皮水解物的特性分析

Honoka Miyamoto, Erwina Safitri, Takeshi Nagai, Masataka Saito
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引用次数: 0

摘要

二肽基肽酶 IV(DPP-IV)抑制剂是一种可降低血糖水平的抗糖尿病药物。关于从废弃鱼皮中提取的肽的 DPP-IV 抑制活性的报道仍然很少。因此,我们利用六种鱼皮制备了各种酶水解物,并研究了它们的 DPP-IV 抑制作用。结果发现,阿尔卡拉酶对黄鱼水解物的 DPP-IV 抑制活性高于其他酶。此外,还发现黄鱼和鳗鱼皮水解物经乙醇分馏后的 IC50 值较低。氨基酸组成分析表明,从鰤鱼皮中获得的水解物含有最多的 Gly,其次是 Pro、Hyp 和 Ala,这表明它是从 I 型胶原蛋白中提取的肽。乙醇分馏显示,DPP-IV 抑制成分包含在低分子量部分。人工消化试验没有观察到 DPP-IV 抑制活性或平均分子量的变化。从鱼皮中提取的 DPP-IV 抑制肽具有作为食品原料应用于各种食品的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Fish Skin Hydrolysates Exhibiting Dipeptidyl Peptidase IV Inhibitory Activity
Dipeptidyl peptidase IV (DPP-IV) inhibitors are antidiabetic drugs that can lower blood sugar levels. There are still few reports on the DPP-IV inhibitory activity of peptides obtained from discarded fish skin. Therefore, we prepared various enzymatic hydrolysates using the skins of six fish species and investigated their DPP-IV inhibitory effects. As a result, it was found that the DPP-IV inhibitory activity of yellowtail hydrolysate by Alcalase was higher than that of other enzymes. In addition, the IC50 after ethanol fractionation was found to be lower in yellowtail and eel skin hydrolysate. Amino acid composition analysis showed that the hydrolysate obtained from the skin of the yellowtail contained the highest amount of Gly, followed by Pro, Hyp, and Ala, indicating that it was a peptide derived from type I collagen. Fractionation with ethanol showed that the DPP-IV inhibitory components were contained in the low molecular weight fraction. The artificial digestion test observed no DPP-IV inhibitory activity or average molecular weight change. The DPP-IV inhibitory peptide obtained from fish skin has the potential to be applied as a food material to various food products.
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