Cnidoscolus quercifolius Pohl 茎皮提取物的抗菌和调节活性化学成分简介

Q2 Pharmacology, Toxicology and Pharmaceutics

Current Bioactive Compounds Pub Date : 2024-05-13 DOI:10.2174/0115734072297726240430040317

Joice Barbosa do Nascimento, José Walber Gonçalves Castro, Maria Inácio da Silva, José Jonas Ferreira Viturino, Mariana Pereira da Silva, Ana Cecilia Calixto Donelardy, Johnatan Wellisson da Silva Mendes, Fabíola Fernandes Galvão Rodrigues, José Galberto Martins da Costa

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引用次数: 0

摘要

本研究旨在研究槲寄生茎皮甲醇提取物和乙酸乙酯的化学成分，并评估其对标准菌株和多重耐药菌株的抗菌和抗生素调节活性。实验结果表明，该提取物仅对产生广谱β-内酰胺酶的肺炎克雷伯菌、肺炎克雷伯菌碳青霉烯酶和大肠埃希菌 ATCC 25922 有抑制作用，抑制浓度分别为 256、512 和 512 μg/mL。这些提取物与庆大霉素和阿莫西林合用时，对所有标准菌株和多重耐药菌株都有协同作用，但肺炎克雷伯菌碳青霉烯酶和产生广谱β-内酰胺酶的肺炎克雷伯菌除外。当与阿米卡星联合使用时，它们还对耐多药菌株产生协同作用；然而，只有乙酸乙酯提取物对大肠杆菌 ATCC 25922 具有协同作用。青霉素与甲醇提取物的结合仅对产生广谱β-内酰胺酶的肺炎克雷伯菌和金黄色葡萄球菌 ATCC 29213 有协同作用，而乙酸乙酯提取物对金黄色葡萄球菌和产生广谱β-内酰胺酶的肺炎克雷伯菌有协同作用。不过，需要使用特定方法对携带不同抗药性机制的细菌（尤其是革兰氏阳性菌）进行进一步研究，以阐明其作用机制。不过，该物种还需要使用特定方法对携带不同抗药性机制的细菌（尤其是革兰氏阳性细菌）进行进一步研究，以阐明其作用机制。

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Chemical Profile with Antibacterial and Modulatory Activity of Extracts from the Stem Bark of Cnidoscolus quercifolius Pohl.

The present study aimed to investigate the chemical profile and evaluate the antibacterial and antibiotic-modulating activity of the methanolic extract and ethyl acetate of the stem bark of C. quercifolius against standard and multiresistant strains. The Minimum Inhibitory Concentration was determined by the microdilution method, and the modulating potential of the extracts on aminoglycoside and β-lactam antibiotics was evaluated. The classes of secondary metabolites identified from qualitative chemical prospecting were flavones, flavonols, xanthones, flavonones, flavonols, catechins and leucoanthocyanidins. The assays showed inhibition only for Klebsiella pneumoniae-producing extended-spectrum β- lactamase, Klebsiella pneumoniae carbapenemase and Escherichia coli ATCC 25922 with MICs of 256, 512 and 512 μg/mL, respectively. The extracts, when associated with gentamicin and amoxicillin, showed synergism against all standard and multiresistant strains, with the exception of Klebsiella pneumoniae carbapenemase and Klebsiella pneumoniae producing extendedspectrum β-lactamase, respectively. When combined with amikacin, they also demonstrated synergism against multidrug-resistant strains; however, only the ethyl acetate extract had a synergistic effect against E. coli ATCC 25922. The association of penicillin with methanolic extract showed synergism only for Klebsiella pneumoniae producing extended-spectrum β-lactamase and S. aureus ATCC 29213, while the ethyl acetate extracts demonstrated synergism against S. aureus and Klebsiella pneumoniae producing extended-spectrum β-lactamase. It was found that C. quercifolius extracts have antibacterial potential against gramnegative bacteria. However, the need for additional research using specific methods against bacteria carrying different resistance mechanisms, especially gram-positive ones, is highlighted, with the aim of elucidating their mechanism of action. The species was found to have substances with potential against Gram-negative bacteria. However, the need for additional research using specific methods against bacteria carrying different resistance mechanisms, especially Gram-positive ones, is highlighted, with the aim of elucidating their mechanism of action.

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来源期刊

Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.90

自引率

0.00%

发文量

112

期刊介绍： The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.