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引用次数: 0
摘要
蛋白 A 亲和层析已被广泛用于重组抗体/Fc 融合纯化的初始产物捕获。然而,一般来说,蛋白 A 缺乏分离聚集体的能力(除非聚集体太大,无法进入树脂珠的孔中,或者其蛋白 A 结合位点被埋没,在这种情况下,聚集体不会结合)。在目前的工作中,我们证明了 CaptureSelect FcXP 亲和培养基具有很强的聚集体分离能力,并在两种双特异性抗体(bsAb)的 pH 或电导梯度洗脱条件下有效地去除了聚集体。在这两种情况下,聚集体含量分别从 >16% 和 >22%(进料)降至
CaptureSelect FcXP affinity medium exhibits strong aggregate separation capability.
Protein A affinity chromatography has been widely used for initial product capture in recombinant antibody/Fc-fusion purification. However, in general Protein A lacks the capability of separating aggregates (unless the aggregates are too large to enter the pores of resin beads or have their Protein A binding sites buried, in which case the aggregates do not bind). In the current work, we demonstrated that CaptureSelect FcXP affinity medium exhibited strong aggregate separation capability and effectively removed aggregates under pH or conductivity gradient elution in two bispecific antibody (bsAb) cases. For these two cases, aggregate contents were reduced from >16% and >22% (in the feed) to <1% and <5% (in the eluate) for the first and second bsAbs, respectively. While more case studies are required to further demonstrate FcXP's superiority in aggregate removal, findings from the current study suggest that FcXP can potentially be a better alternative than Protein A for product capture in cases where aggregate content is high.
期刊介绍:
Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field. The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods. However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.