利用串联质量标记(TMT)和平行反应监测(PRM)质谱对感染了天牛埃默氏菌的鸡 DF-1 细胞进行定量磷蛋白组学分析。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-17 DOI:10.1051/parasite/2024027
Liu-Shu Jia, Zhan Liu, Shun-Hai Zhu, Qi-Ping Zhao, Hong-Yu Han, Huan-Zhi Zhao, Yu Yu, Hui Dong
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引用次数: 0

摘要

天牛埃默氏菌是一种细胞内寄生虫,对家禽养殖业造成了巨大危害。蛋白质磷酸化在宿主细胞与天牛埃默氏菌的相互作用中起着至关重要的作用。然而,目前还没有发表过对宿主细胞在天牛埃希氏菌感染的不同阶段进行全面的磷酸化蛋白质组分析。在本研究中,我们对未感染(UI)或感染天牛星虫 6 小时(PI6,早期入侵阶段)或 36 小时(PI36,滋养体发育阶段)的鸡胚 DF-1 成纤维细胞进行了定量磷酸化蛋白质组学分析。共鉴定出 10,122 个磷酸肽与 3,398 个宿主细胞磷酸蛋白相匹配,并鉴定出 13,437 个磷酸化位点。其中,在 PI6/UI、PI36/UI 和 PI36/PI6 比较中分别鉴定出 491、1,253 和 275 个差异表达的磷酸化蛋白。KEGG通路富集分析表明,天牛血吸虫通过磷酸化调控宿主细胞过程,包括支持其早期入侵阶段的病灶粘附、肌动蛋白细胞骨架调控和FoxO信号转导,以及有利于滋养体发育的粘附连接和ErbB信号转导通路。这些结果丰富了天牛血吸虫与宿主细胞相互作用的数据,有助于更好地理解宿主与寄生虫关系的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantitative phosphoproteomic analysis of chicken DF-1 cells infected with Eimeria tenella, using tandem mass tag (TMT) and parallel reaction monitoring (PRM) mass spectrometry.

Eimeria tenella is an obligate intracellular parasite which causes great harm to the poultry breeding industry. Protein phosphorylation plays a vital role in host cell-E. tenella interactions. However, no comprehensive phosphoproteomic analyses of host cells at various phases of E. tenella infection have been published. In this study, quantitative phosphoproteomic analysis of chicken embryo DF-1 fibroblasts that were uninfected (UI) or infected with E. tenella for 6 h (PI6, the early invasion phase) or 36 h (PI36, the trophozoite development phase) was conducted. A total of 10,122 phosphopeptides matched to 3,398 host cell phosphoproteins were identified and 13,437 phosphorylation sites were identified. Of these, 491, 1,253, and 275 differentially expressed phosphorylated proteins were identified in the PI6/UI, PI36/UI, and PI36/PI6 comparisons, respectively. KEGG pathway enrichment analysis showed that E. tenella modulated host cell processes through phosphorylation, including focal adhesion, regulation of the actin cytoskeleton, and FoxO signaling to support its early invasion phase, and modulating adherens junctions and the ErbB signaling pathway to favor its trophozoite development. These results enrich the data on the interaction between E. tenella and host cells and facilitate a better understanding of the molecular mechanisms underlying host-parasite relationships.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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