利用磁化多壁碳纳米管上的固定化漆酶对稻草进行糖化。

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI:10.1007/s10529-024-03494-z
Hasnol Azahari Natasha Yasmin, Balakrishnan Kunasundari, Siew Hoong Shuit, Mohamad Fahrurrazi Tompang
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引用次数: 0

摘要

利用磁性载体有效回收固定化酶,使人们对这项技术的兴趣与日俱增。本研究的目的是评估固定漆酶在磁化多壁碳纳米管(m-MWCNTs)上的稳定性和可重复使用性。漆酶被有效地吸附在用水合成的磁化多壁碳纳米管(m-MWCNTs)上。7 毫克拉克酶/毫升的浓度是理想的固定化浓度。在 pH 值为 5 时,游离和固定化的漆酶都具有最佳活性,而后者的最佳温度则从 40 ℃ 提高到了 50 ℃。与游离漆酶相比,固定化漆酶在更极端的温度下表现出更大范围的稳定性。在第四个反应循环中,固定化漆酶表现出超过 60% 的相对活性,可以重复使用。根据傅立叶变换红外光谱(FTIR)在 2921 cm-1 处的峰值,使用固定化漆酶对稻草进行糖化验证了木质素降解。固定化漆酶在 m-MWCNTs 上的易回收性使其有望用于生物质水解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes.

Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes.

The effective recovery of the immobilized enzymes using magnetic carriers has led to growing interest in this technology. The objective of this research was to evaluate the efficiency of immobilized laccase on magnetized multiwall carbon nanotubes (m-MWCNTs) in terms of stability and reusability. Laccases were efficiently adsorbed onto magnetized multiwall carbon nanotubes (m-MWCNTs) synthesized using water. The concentration of 7 mg laccase/mL was found to be ideal for immobilization. The optimal activity of both free and immobilized laccases was observed at pH 5, while for the latter, the optimal temperature was shifted from 40 to 50 °C. Compared to the free laccase, the immobilized laccase exhibited a greater range of stability at more extreme temperatures. At the fourth cycle of reactions, the immobilized laccase exhibited more than 60% relative activity in terms of reusability. Based on the fourier-transform infrared spectroscopy (FTIR) peak at 2921 cm-1, saccharification of paddy straw using immobilized laccase verified lignin degradation. The easy recovery of the immobilized laccase on m-MWCNTs lends credence to its potential use in biomass hydrolysis.

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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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