通过移液管内毛细管延迟递送格列宁可促进穿孔贴片记录。

IF 2.4 4区医学 Q3 NEUROSCIENCES

Neuroscience Research Pub Date : 2024-05-11 DOI:10.1016/j.neures.2024.05.002

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引用次数: 0

摘要

要在不改变细胞内 Cl- 浓度的情况下记录神经元活动，gramicidin 穿孔膜片钳技术是不可或缺的。传统上，移液管液中所含的gramicidin是通过被动扩散输送到细胞膜上的。沉积在移液管口上的gramicidin有时会阻碍千兆位密封的形成，而且穿孔的速度很慢。这些问题可以通过在千兆封口形成后从移液管内毛细管输送高浓度的gramicidin来解决。我们在此介绍这种改进方法的详细方案。这种方法将极大地促进对依赖于 Cl- 梯度的神经元活动的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Delayed gramicidin delivery through an intra-pipette capillary facilitates perforated patch recordings

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Delayed gramicidin delivery through an intra-pipette capillary facilitates perforated patch recordings

The gramicidin-perforated patch-clamp technique is indispensable for recording neuronal activities without changing the intracellular Cl⁻ concentration. Conventionally, gramicidin contained in the pipette fluid is delivered to the cell membrane by passive diffusion. Gramicidin deposited on the pipette orifice sometimes hampers giga-seal formation, and perforation progresses only slowly. These problems may be circumvented by delivering a high concentration of gramicidin from an intra-pipette capillary after a giga-seal is formed. We herein describe the detailed protocol of this improved method. This protocol would greatly facilitate the investigation of Cl⁻ gradient-dependent neuronal activities.

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来源期刊

Neuroscience Research 医学-神经科学

CiteScore

5.60

自引率

3.40%

发文量

136

审稿时长

28 days

期刊介绍： The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.