利用基于血清素的 X 射线染色和高分辨率 3D 显微 CT 评估软骨肉瘤:一项可行性研究。

IF 3.7 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Alexandra S Gersing, Melanie A Kimm, Christine Bollwein, Patrick Ilg, Carolin Mogler, Felix G Gassert, Georg C Feuerriegel, Carolin Knebel, Klaus Woertler, Daniela Pfeiffer, Madleen Busse, Franz Pfeiffer
{"title":"利用基于血清素的 X 射线染色和高分辨率 3D 显微 CT 评估软骨肉瘤:一项可行性研究。","authors":"Alexandra S Gersing, Melanie A Kimm, Christine Bollwein, Patrick Ilg, Carolin Mogler, Felix G Gassert, Georg C Feuerriegel, Carolin Knebel, Klaus Woertler, Daniela Pfeiffer, Madleen Busse, Franz Pfeiffer","doi":"10.1186/s41747-024-00454-0","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Chondrosarcomas are rare malignant bone tumors diagnosed by analyzing radiological images and histology of tissue biopsies and evaluating features such as matrix calcification, cortical destruction, trabecular penetration, and tumor cell entrapment.</p><p><strong>Methods: </strong>We retrospectively analyzed 16 cartilaginous tumor tissue samples from three patients (51-, 54-, and 70-year-old) diagnosed with a dedifferentiated chondrosarcoma at the femur, a moderately differentiated chondrosarcoma in the pelvis, and a predominantly moderately differentiated chondrosarcoma at the scapula, respectively. We combined a hematein-based x-ray staining with high-resolution three-dimensional (3D) microscopic x-ray computed tomography (micro-CT) for nondestructive 3D tumor assessment and tumor margin evaluation.</p><p><strong>Results: </strong>We detected trabecular entrapment on 3D micro-CT images and followed bone destruction throughout the volume. In addition to staining cell nuclei, hematein-based staining also improved the visualization of the tumor matrix, allowing for the distinction between the tumor and the bone marrow cavity. The hematein-based staining did not interfere with further conventional histology. There was a 5.97 ± 7.17% difference between the relative tumor area measured using micro-CT and histopathology (p = 0.806) (Pearson correlation coefficient r = 0.92, p = 0.009). Signal intensity in the tumor matrix (4.85 ± 2.94) was significantly higher in the stained samples compared to the unstained counterparts (1.92 ± 0.11, p = 0.002).</p><p><strong>Conclusions: </strong>Using nondestructive 3D micro-CT, the simultaneous visualization of radiological and histopathological features is feasible.</p><p><strong>Relevance statement: </strong>3D micro-CT data supports modern radiological and histopathological investigations of human bone tumor specimens. It has the potential for being an integrative part of clinical preoperative diagnostics.</p><p><strong>Key points: </strong>• Matrix calcifications are a relevant diagnostic feature of bone tumors. • Micro-CT detects all clinically diagnostic relevant features of x-ray-stained chondrosarcoma. • Micro-CT has the potential to be an integrative part of clinical diagnostics.</p>","PeriodicalId":36926,"journal":{"name":"European Radiology Experimental","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089022/pdf/","citationCount":"0","resultStr":"{\"title\":\"Chondrosarcoma evaluation using hematein-based x-ray staining and high-resolution 3D micro-CT: a feasibility study.\",\"authors\":\"Alexandra S Gersing, Melanie A Kimm, Christine Bollwein, Patrick Ilg, Carolin Mogler, Felix G Gassert, Georg C Feuerriegel, Carolin Knebel, Klaus Woertler, Daniela Pfeiffer, Madleen Busse, Franz Pfeiffer\",\"doi\":\"10.1186/s41747-024-00454-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Chondrosarcomas are rare malignant bone tumors diagnosed by analyzing radiological images and histology of tissue biopsies and evaluating features such as matrix calcification, cortical destruction, trabecular penetration, and tumor cell entrapment.</p><p><strong>Methods: </strong>We retrospectively analyzed 16 cartilaginous tumor tissue samples from three patients (51-, 54-, and 70-year-old) diagnosed with a dedifferentiated chondrosarcoma at the femur, a moderately differentiated chondrosarcoma in the pelvis, and a predominantly moderately differentiated chondrosarcoma at the scapula, respectively. We combined a hematein-based x-ray staining with high-resolution three-dimensional (3D) microscopic x-ray computed tomography (micro-CT) for nondestructive 3D tumor assessment and tumor margin evaluation.</p><p><strong>Results: </strong>We detected trabecular entrapment on 3D micro-CT images and followed bone destruction throughout the volume. In addition to staining cell nuclei, hematein-based staining also improved the visualization of the tumor matrix, allowing for the distinction between the tumor and the bone marrow cavity. The hematein-based staining did not interfere with further conventional histology. There was a 5.97 ± 7.17% difference between the relative tumor area measured using micro-CT and histopathology (p = 0.806) (Pearson correlation coefficient r = 0.92, p = 0.009). Signal intensity in the tumor matrix (4.85 ± 2.94) was significantly higher in the stained samples compared to the unstained counterparts (1.92 ± 0.11, p = 0.002).</p><p><strong>Conclusions: </strong>Using nondestructive 3D micro-CT, the simultaneous visualization of radiological and histopathological features is feasible.</p><p><strong>Relevance statement: </strong>3D micro-CT data supports modern radiological and histopathological investigations of human bone tumor specimens. It has the potential for being an integrative part of clinical preoperative diagnostics.</p><p><strong>Key points: </strong>• Matrix calcifications are a relevant diagnostic feature of bone tumors. • Micro-CT detects all clinically diagnostic relevant features of x-ray-stained chondrosarcoma. • Micro-CT has the potential to be an integrative part of clinical diagnostics.</p>\",\"PeriodicalId\":36926,\"journal\":{\"name\":\"European Radiology Experimental\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-05-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11089022/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Radiology Experimental\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s41747-024-00454-0\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Radiology Experimental","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s41747-024-00454-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
引用次数: 0

摘要

背景:软骨肉瘤是一种罕见的恶性骨肿瘤:软骨肉瘤是一种罕见的恶性骨肿瘤,通过分析组织活检的放射影像和组织学,评估基质钙化、皮质破坏、骨小梁穿透和肿瘤细胞嵌顿等特征来诊断:我们回顾性分析了三位患者(51、54 和 70 岁)的 16 份软骨肿瘤组织样本,他们分别被诊断为股骨部位的去分化软骨肉瘤、骨盆部位的中度分化软骨肉瘤和肩胛骨部位的主要中度分化软骨肉瘤。我们将基于血色素的X射线染色与高分辨率三维(3D)显微X射线计算机断层扫描(micro-CT)相结合,进行无损三维肿瘤评估和肿瘤边缘评价:我们在三维显微计算机断层扫描图像上检测到了骨小梁夹层,并跟踪了整个体积内的骨破坏情况。除了对细胞核进行染色外,基于血色素的染色还能改善肿瘤基质的可视化,从而区分肿瘤和骨髓腔。血色素染色不会干扰进一步的常规组织学检查。使用 micro-CT 和组织病理学测量的相对肿瘤面积相差 5.97 ± 7.17%(p = 0.806)(皮尔逊相关系数 r = 0.92,p = 0.009)。染色样本的肿瘤基质信号强度(4.85 ± 2.94)明显高于未染色样本(1.92 ± 0.11,p = 0.002):结论:使用无损三维显微 CT,可同时观察放射学和组织病理学特征:三维显微 CT 数据支持对人类骨肿瘤标本进行现代放射学和组织病理学研究。它有可能成为临床术前诊断的一个综合部分:- 要点:基质钙化是骨肿瘤的一个相关诊断特征。- Micro-CT可检测出X光染色软骨肉瘤的所有临床诊断相关特征。- 显微 CT 有可能成为临床诊断的一个组成部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chondrosarcoma evaluation using hematein-based x-ray staining and high-resolution 3D micro-CT: a feasibility study.

Chondrosarcoma evaluation using hematein-based x-ray staining and high-resolution 3D micro-CT: a feasibility study.

Background: Chondrosarcomas are rare malignant bone tumors diagnosed by analyzing radiological images and histology of tissue biopsies and evaluating features such as matrix calcification, cortical destruction, trabecular penetration, and tumor cell entrapment.

Methods: We retrospectively analyzed 16 cartilaginous tumor tissue samples from three patients (51-, 54-, and 70-year-old) diagnosed with a dedifferentiated chondrosarcoma at the femur, a moderately differentiated chondrosarcoma in the pelvis, and a predominantly moderately differentiated chondrosarcoma at the scapula, respectively. We combined a hematein-based x-ray staining with high-resolution three-dimensional (3D) microscopic x-ray computed tomography (micro-CT) for nondestructive 3D tumor assessment and tumor margin evaluation.

Results: We detected trabecular entrapment on 3D micro-CT images and followed bone destruction throughout the volume. In addition to staining cell nuclei, hematein-based staining also improved the visualization of the tumor matrix, allowing for the distinction between the tumor and the bone marrow cavity. The hematein-based staining did not interfere with further conventional histology. There was a 5.97 ± 7.17% difference between the relative tumor area measured using micro-CT and histopathology (p = 0.806) (Pearson correlation coefficient r = 0.92, p = 0.009). Signal intensity in the tumor matrix (4.85 ± 2.94) was significantly higher in the stained samples compared to the unstained counterparts (1.92 ± 0.11, p = 0.002).

Conclusions: Using nondestructive 3D micro-CT, the simultaneous visualization of radiological and histopathological features is feasible.

Relevance statement: 3D micro-CT data supports modern radiological and histopathological investigations of human bone tumor specimens. It has the potential for being an integrative part of clinical preoperative diagnostics.

Key points: • Matrix calcifications are a relevant diagnostic feature of bone tumors. • Micro-CT detects all clinically diagnostic relevant features of x-ray-stained chondrosarcoma. • Micro-CT has the potential to be an integrative part of clinical diagnostics.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
European Radiology Experimental
European Radiology Experimental Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
6.70
自引率
2.60%
发文量
56
审稿时长
18 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信