一种可重复且经济实惠的荧光素酶检测方法。

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-05-10 DOI:10.1016/j.ab.2024.115558

Kirtana Reddy, Balaji Ramachandran

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引用次数: 0

摘要

市售荧光素酶检测试剂盒广受欢迎，使用方便。然而，在高通量筛选方面，商业试剂盒因运行成本高昂而受到限制。作为商业荧光素酶检测试剂盒的替代品，本研究提出了一种经济有效的方法，用于进行简单快速的实验室荧光素酶检测。所提出的荧光素酶检测方法用途广泛，既可以在微孔板阅读器中对裂解液进行定量检测筛选，也可以在成像系统下对活细胞进行定性或定量筛选。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A reproducible and affordable method of conducting luciferase assay

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A reproducible and affordable method of conducting luciferase assay

Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.