智能手机显微镜成像和量化饮用水中微塑料的方法。

IF 2 3区 工程技术 Q2 ANATOMY & MORPHOLOGY
Asmita Karki, Bishan Man Thaiba, K. C. Shishir Acharya, Thakur Sedai, Baburam Kandel, Hari Paudyal, Khaga Raj Sharma, Basant Giri, Bhanu Bhakta Neupane
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引用次数: 0

摘要

饮用水中的微塑料由于粒径较小,颗粒数量较少,因此分析起来往往具有挑战性。在这项研究中,我们使用了一种成本低廉、易于组装的智能手机显微系统,对小至 20 μm 的微塑料颗粒进行成像和定量。该系统由一个直径为 4 毫米的球形蓝宝石球透镜和一个智能手机摄像头组成,后者是主要的成像组件。该系统还包括使用氯化锌溶液对样品进行预浓缩。该方法在过滤蒸馏水和去离子水样品(n = 9)中的峰值回收率和检出限分别为 55.6% ± 9.7% 和 34 微粒/升。显微系统的成像性能与商用明视野显微系统相似。该方法进一步用于检测商用瓶装水和罐装水中的微塑料颗粒(n = 20)。瓶装水和罐装水样品中的微粒数量分别为 0-91 微粒/升和 0-130 微粒/升。在这两种水样中都观察到了不同形状和大小的颗粒。傅立叶变换红外光谱(n = 36)进一步确认了从水样中收集到的微粒,发现 97% 的测试微粒由塑料材料制成。这些研究结果表明,智能手机显微系统可作为一种低成本的替代方法,用于初步筛查饮用水样本中的微塑料。研究亮点使用基于球透镜的智能手机显微镜方法进行微塑料分析。在瓶装水和罐装水样本中发现了不同形状和大小的颗粒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Smartphone microscopic method for imaging and quantification of microplastics in drinking water

Smartphone microscopic method for imaging and quantification of microplastics in drinking water

Analysis of microplastics in drinking water is often challenging due to smaller particle size and low particle count. In this study, we used a low cost and an easy to assemble smartphone microscopic system for imaging and quantitating microplastic particles as small as 20 μm. The system consisted of a spherical sapphire ball lens of 4 mm diameter attached to a smartphone camera as a major imaging component. It also involved pre-concentration of the sample using ZnCl2 solution. The spike recovery and limit of detection of the method in filtered distilled and deionized water samples (n = 9) were 55.6% ± 9.7% and 34 particles/L, respectively. Imaging performance of the microscopic system was similar to a commercial bright field microscopic system. The method was further implemented to examine microplastic particles in commercial bottled and jar water samples (n = 20). The particles count in bottled and jar water samples ranged from 0–91 particles/L to 0–130 particles/L, respectively. In both sample types, particles of diverse shape and size were observed. The particles collected from water samples were further confirmed by FTIR spectra (n = 36), which found 97% of the particles tested were made of plastic material. These findings suggested that the smartphone microscopic system can be implemented as a low-cost alternative for preliminary screening of microplastic in drinking water samples.

Research Highlights

  • Ball lens based smartphone microscopic method was used for microplastic analysis.
  • Particles of diverse shape and size were found in bottle and jar water samples.
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来源期刊
Microscopy Research and Technique
Microscopy Research and Technique 医学-解剖学与形态学
CiteScore
5.30
自引率
20.00%
发文量
233
审稿时长
4.7 months
期刊介绍: Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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