O. V. Danilova, I. Y. Oshkin, R. S. Suleimanov, S. N. Dedysh
{"title":"螺旋形甲烷菌 \"Methylospira mobilis \"新菌株的表型特征和基因组分析","authors":"O. V. Danilova, I. Y. Oshkin, R. S. Suleimanov, S. N. Dedysh","doi":"10.1134/s0026261723603627","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Taxon “<i>Methylospira mobilis</i>” accommodates micro-aerobic, spiral-shaped methanotrophic bacteria, which are widely distributed in low-oxygen habitats. Due to difficulties of cultivation, these methanotrophs remained represented by a single isolate, strain Shm1, which was obtained from a boreal peat bog of European Russia. This study reports isolation of a novel member of this candidate genus, strain SPMX, obtained from a subarctic wetland. The 16S rRNA gene sequence of strain SPMX was identical to that in strain Shm1. The complete genome sequence of strain SPMX was 4.6 Mb in size and displayed 99.5% average nucleotide identity to the genome sequence of strain Shm1, thus suggesting affiliation of these bacteria to one methanotroph species. In contrast to strain Shm1, the new isolate was more tolerant to low temperatures and was less growth-dependent on the presence of СО<sub>2</sub>. The genomes of both spiral-shaped methanotrophs encoded form II ribulose bisphosphate carboxylase, which makes them different from the majority of other type X methanotrophs that possess form I of this enzyme.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"5 1","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotypic Characterization and Genome Analysis of a Novel Isolate of the Spiral-Shaped Methanotroph “Methylospira mobilis”\",\"authors\":\"O. V. Danilova, I. Y. Oshkin, R. S. Suleimanov, S. N. Dedysh\",\"doi\":\"10.1134/s0026261723603627\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p>Taxon “<i>Methylospira mobilis</i>” accommodates micro-aerobic, spiral-shaped methanotrophic bacteria, which are widely distributed in low-oxygen habitats. Due to difficulties of cultivation, these methanotrophs remained represented by a single isolate, strain Shm1, which was obtained from a boreal peat bog of European Russia. This study reports isolation of a novel member of this candidate genus, strain SPMX, obtained from a subarctic wetland. The 16S rRNA gene sequence of strain SPMX was identical to that in strain Shm1. The complete genome sequence of strain SPMX was 4.6 Mb in size and displayed 99.5% average nucleotide identity to the genome sequence of strain Shm1, thus suggesting affiliation of these bacteria to one methanotroph species. In contrast to strain Shm1, the new isolate was more tolerant to low temperatures and was less growth-dependent on the presence of СО<sub>2</sub>. The genomes of both spiral-shaped methanotrophs encoded form II ribulose bisphosphate carboxylase, which makes them different from the majority of other type X methanotrophs that possess form I of this enzyme.</p>\",\"PeriodicalId\":18514,\"journal\":{\"name\":\"Microbiology\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-02-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1134/s0026261723603627\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0026261723603627","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Phenotypic Characterization and Genome Analysis of a Novel Isolate of the Spiral-Shaped Methanotroph “Methylospira mobilis”
Abstract
Taxon “Methylospira mobilis” accommodates micro-aerobic, spiral-shaped methanotrophic bacteria, which are widely distributed in low-oxygen habitats. Due to difficulties of cultivation, these methanotrophs remained represented by a single isolate, strain Shm1, which was obtained from a boreal peat bog of European Russia. This study reports isolation of a novel member of this candidate genus, strain SPMX, obtained from a subarctic wetland. The 16S rRNA gene sequence of strain SPMX was identical to that in strain Shm1. The complete genome sequence of strain SPMX was 4.6 Mb in size and displayed 99.5% average nucleotide identity to the genome sequence of strain Shm1, thus suggesting affiliation of these bacteria to one methanotroph species. In contrast to strain Shm1, the new isolate was more tolerant to low temperatures and was less growth-dependent on the presence of СО2. The genomes of both spiral-shaped methanotrophs encoded form II ribulose bisphosphate carboxylase, which makes them different from the majority of other type X methanotrophs that possess form I of this enzyme.
期刊介绍:
Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.