曲妥珠单抗单链 Fab 片段的骨架和甲基侧链共振分配。

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2024-05-08 DOI:10.1007/s12104-024-10177-3

Donald Gagné, James M. Aramini, Yves Aubin

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引用次数: 0

摘要

曲妥珠单抗是一种治疗性单克隆抗体，主要针对早期癌症中含量较高的人类表皮生长因子受体 2（HER2）。在此，我们报告了曲妥珠单抗-scFab 片段骨架和异亮氨酸、亮氨酸和缬氨酸残基甲基的近乎完整的共振分配及其立体分配。该抗体片段是在大肠杆菌中采用单链方法制备的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Backbone and methyl side-chain resonance assignments of the single chain Fab fragment of trastuzumab

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Backbone and methyl side-chain resonance assignments of the single chain Fab fragment of trastuzumab

Trastuzumab is a therapeutic monoclonal antibody developed to target human epidermal growth factor receptor 2 (HER2) present at higher levels in early cancers. Here we report the near complete resonance assignment of trastuzumab-scFab fragment backbone and the methyl groups of isoleucine, leucine and valine residues, as well as their stereo-assignments. The antibody fragment was produced using a single chain approach in Escherichia coli.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.