利用人体诱导多能干细胞生成附肢皮肤器官组织的优化方案。

IF 2.5 Q3 BIOCHEMICAL RESEARCH METHODS

Biology Methods and Protocols Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI:10.1093/biomethods/bpae019

Imaan Ahmed, Jane Sun, Jason Brown, Kiarash Khosrotehrani, Abbas Shafiee

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引用次数: 0

摘要

由多能干细胞生成类器官是一项尖端技术，它为体外模拟人体器官创造了新的可能性，并为再生医学开辟了道路。在这里，我们介绍一种通过直接胚状体形成从人类诱导多能干细胞（hiPSCs）生成皮肤器官组织（SKOs）的方法。这种方法为hiPSC分化提供了一个一致的起点，使来自两种不同体细胞来源的hiPSC品系产生的SKO具有复杂的皮肤结构和附属物（如毛囊、皮脂腺等）。

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An optimized protocol for generating appendage-bearing skin organoids from human-induced pluripotent stem cells.

Organoid generation from pluripotent stem cells is a cutting-edge technique that has created new possibilities for modelling human organs in vitro, as well as opening avenues for regenerative medicine. Here, we present a protocol for generating skin organoids (SKOs) from human-induced pluripotent stem cells (hiPSCs) via direct embryoid body formation. This method provides a consistent start point for hiPSC differentiation, resulting in SKOs with complex skin architecture and appendages (e.g. hair follicles, sebaceous glands, etc.) across hiPSC lines from two different somatic sources.

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来源期刊

Biology Methods and Protocols Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

3.80

自引率

2.80%

发文量

审稿时长

19 weeks