冷冻保存的人类精子中的水蒸发蛋白表达:探索天然深共晶溶剂(NADEs)的能力。

IF 1 4区 生物学 Q3 BIOLOGY
Cryo letters Pub Date : 2024-05-01
F Jahanseir, F Ghasemian, Z Zahiri
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引用次数: 0

摘要

背景:水蒸发蛋白(AQPs)是冷冻保存过程中促进水和冷冻保护剂(CPAs)快速移动的重要蛋白质,可确保精子细胞的冷冻耐受性:本研究评估了使用天然深共晶溶剂(NADES)作为冷冻保护剂的人类精子在经历冷冻后水蒸素水平的保存情况:2021 年 6 月至 2022 年 10 月,伊朗拉什特 Mehr 不育症治疗研究所采集了 35 份精子参数正常的精液样本。这些样本被分成几组进行分析:对照组(未冷冻)、用 SpermFreeze 溶液冷冻的组和用不同 NADES(包括 ChS、ChX、ChU、ChG、GlyP 和 EtP)冷冻的组。解冻后,对各组精子进行多方面评估,包括精子染色质的完整性和凝集、活力、运动能力、顶体的完整性以及AQP1、AQP3、AQP7、AQP8和AQP9基因的表达:基因表达分析表明,与对照组相比,冷冻 ChS 和 GlyP 可保持 AQP1 和 AQP3 基因的表达。至于 AQP7 和 AQP8,某些 NADES 组(如 ChS、ChU 和 GlyP)与对照组的表达水平没有明显差异。此外,使用特定 NADES(如 ChS、ChG、EtP 和 GlyP)冷冻的样本与对照组相比,AQP9 的表达水平有所保留:这些发现强调了NADES在保存冷冻保存的人类精子中的水通道蛋白表达及其重要生育参数方面的重要性。Doi.org/10.54680/fr24310110512.
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aquaporin expression in cryopreserved human sperm: exploring the capabilities of natural deep eutectic solvents (NADEs).

Background: Aquaporins (AQPs) are essential proteins that facilitate the rapid movement of water and cryoprotective agents (CPAs) during the cryopreservation process, and ensure the cryo-tolerance of sperm cells.

Objective: This study evaluated the preservation of aquaporin levels in human sperm after undergoing freezing using natural deep eutectic solvents (NADES) as CPAs for cryoprotection.

Materials and methods: From June 2021 to October 2022, 35 semen samples with normal sperm parameters were acquired from the Mehr Infertility Treatment Institute in Rasht, Iran. The samples were divided into several groups for analysis: control group (not frozen), group frozen with SpermFreeze Solution, and groups frozen with different NADESs, including ChS, ChX, ChU, ChG, GlyP, and EtP. After thawing, various aspects for each group were assessed, including the integrity and condensation of sperm chromatin, viability, motility, integrity of acrosome, and the expression of AQP1, AQP3, AQP7, AQP8, and AQP9 genes.

Results: The analysis of gene expression revealed that freezing with ChS and GlyP preserved the expression of the AQP1 and AQP3 genes compared to the control group. Regarding AQP7 and AQP8, significant differences were not observed in expression levels between certain NADES groups (e.g., ChS, ChU, and GlyP) and the control group. Additionally, samples frozen with specific NADESs, such as ChS, ChG, EtP, and GlyP, exhibited preserved levels of AQP9 expression when compared to the control group.

Conclusion: These findings emphasize the importance of NADES in preserving the expression of aquaporins in cryopreserved human sperm and their important fertility parameters. Doi.org/10.54680/fr24310110512.

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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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