下调的PDIA3P1 lncRNA通过调控PE中的蜗牛和SFRP1损害滋养层细胞表型

IF 2.6 4区 医学 Q3 CELL BIOLOGY
Zhengzheng Ding, Liuxin Wu, Yue Sun, Yuanyuan Zhu, Qing Zuo, Li Yuan, Cong Wang, Lizhou Sun, Yetao Xu, Yuanyuan Zhang
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引用次数: 0

摘要

子痫前期(PE)是一种妊娠期特有的并发症,由滋养细胞侵袭不足导致胎盘功能受损引起。越来越多的证据表明,假基因(长非编码 RNA 的一个子集)在子痫前期的病理过程中起着关键作用。本研究提出了一个新发现,即与正常组织相比,PE 胎盘组织中的假基因 PDIA3P1 明显下调。体外功能测试显示,抑制 PDIA3P1 会阻碍滋养细胞的增殖、侵袭和迁移,同时上调分泌型皱纹相关蛋白 1(SFRP1)的表达。利用人体滋养细胞进一步探讨了 PDIA3P1 在 PE 中的调控作用,结果发现 PDIA3P1 通过与 HuR 结合发挥其功能,从而增强了滋养细胞中 Snail 表达的稳定性。总之,我们的研究结果表明,PDIA3P1 在调节滋养细胞特性和促进 PE 发病机制方面起着至关重要的作用,为预后和治疗干预提供了潜在的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Downregulated PDIA3P1 lncRNA Impairs Trophoblast Phenotype by Regulating Snail and SFRP1 in PE
Preeclampsia (PE) manifests as a pregnancy-specific complication arising from compromised placentation characterized by inadequate trophoblast invasion. A growing body of evidence underscores the pivotal involvement of pseudogenes, a subset of long noncoding RNAs, in the pathological processes of PE. This study presents a novel finding, demonstrating a significant downregulation of the pseudogene PDIA3P1 in PE placental tissues compared to normal tissues. In vitro functional assays revealed that suppressing PDIA3P1 hindered trophoblast proliferation, invasion, and migration, concurrently upregulating the expression of secreted frizzled-related protein 1 (SFRP1). Further exploration of the regulatory role of PDIA3P1 in PE, utilizing human trophoblasts, established that PDIA3P1 exerts its function by binding to HuR, thereby enhancing the stability of Snail expression in trophoblasts. Overall, our findings suggest a crucial role for PDIA3P1 in regulating trophoblast properties and contributing to the pathogenesis of PE, offering potential targets for prognosis and therapeutic intervention.
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来源期刊
Analytical Cellular Pathology
Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY
CiteScore
4.90
自引率
3.10%
发文量
70
审稿时长
16 weeks
期刊介绍: Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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