糖皮质激素受体被肝素激活,被纤溶酶失活。

P Arányi, R Machovich
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引用次数: 0

摘要

鸡胸腺糖皮质激素受体在肝素和/或纤溶酶存在下被激活。在25℃条件下,浓度为9微米的肝素加速了活化速率,但对3h -曲安奈德-受体复合物的最大活化分数没有显著影响。相反,在25℃孵育(激活)前添加0.7微米的纤溶酶阻断了DNA纤维素的结合。凝血酶不影响受体的激活。加入到活化复合体中,纤溶酶导致快速失活,即DNA结合能力的不可逆丧失。纤溶酶和肝素似乎相互独立地发挥作用,尽管它们已知在问题的浓度范围内相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Glucocorticoid receptor is activated by heparin and deactivated by plasmin.

Chick thymus glucocorticoid receptor activation was followed in the presence of heparin and/or plasmin. Heparin, at a concentration of 9 microM, accelerated the rate of activation at 25 degrees C without influencing significantly the maximum activated fraction of the 3H-triamcinolone acetonide-receptor complex. On the contrary, 0.7 microM plasmin added prior to incubation at 25 degrees C (activation) of the complex blocked DNA cellulose binding. Thrombin did not influence receptor activation. Added to the activated complex, plasmin resulted in a rapid deactivation, i.e. an irreversible loss of DNA binding capacity. Plasmin and heparin appeared to exert their effects independent of each other in spite of the fact that they are known to interact in the concentration range in question.

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