蛋鸡输卵管中 NLRC5 的表达谱及其在雌二醇治疗诱导蜕皮鸡后的变化

A. M. Abdel Mageed, B. Ariyadi, N. Isobe, Y. Yoshimura
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引用次数: 0

摘要

先天性免疫防御系统是通过不同家族的受体(如 TLRs 和细胞内 NLR 家族)识别微生物成分而启动的。在 NLR 家族中,NLRC5 能识别微生物的致病成分。本研究旨在确定 NLRC5 在蛋鸡输卵管粘膜组织中的表达谱,并研究输卵管退化和苯甲酸雌二醇(EB)对其表达的影响。在这项研究中,我们使用了产蛋鸡和蜕皮鸡两组。蜕壳组通过肌肉注射单剂量芝麻油或苯甲酸雌二醇进行治疗。为了检测产蛋组、蜕皮组和雌二醇注射组输卵管组织学上的差异,我们将各组输卵管各段的组织样本固定在福尔马林中,并用苏木精和伊红染色。为了观察 NLRC5 基因的轮廓和雌二醇处理的影响,从正常产蛋鸡输卵管全段的表面上皮和固有层,以及蜕皮组的子宫颈、峡部、子宫和阴道的粘膜组织中获取 RNA。粘膜表面上皮和固有层均表达 NLRC5。蜕皮组 NLRC5 的表达量高于产蛋组,而 EB 组则较低。这些结果表明,NLRC5可能在识别入侵产蛋鸡和蜕壳鸡输卵管的病原体方面发挥作用,其表达随输卵管的生长而变化。51(3):345-359, 2023
本文章由计算机程序翻译,如有差异,请以英文原文为准。
NLRC5 expression profile in the oviduct of laying hens and its changes following estradiol treatment in induced molting hens
The innate immune defense system is initiated by recognizing components of microorganisms by different families of receptors such as TLRs and the intracellular NLR family. Among the NLR family, NLRC5 recognizes pathogenic components of microorganisms. The current study aimed to determine the expression profile of NLRC5 in the mucosal tissue of the oviduct of laying hens and to examine the effects of oviduct regression and estradiol benzoate (EB) on its expression. In this study, we used two groups of laying and molting hens. The molting group was treated by receiving a single dose of sesame oil or estradiol benzoate through intramuscular injection. To examine the histological differences in the oviduct between laying, molting, and estradiol-injected groups, Tissue samples from all segments of the oviduct of all groups were fixed in formalin and stained with hematoxylin and eosin. To observe the profile of NLRC5 gene and the effects of estradiol treatment, RNA was obtained from the surface epithelium and lamina propria of the whole segments of the oviduct of normal laying hens, and mucosal tissue of magnum, isthmus, uterus, and the vagina of molting groups. Both mucosal surface epithelium and lamina propria expressed NLRC5. The expression of NLRC5 was higher in the molting than in the laying group, and it was lower in the EB group. These results suggested that NLRC5 may have a role in recognizing pathogens invading the oviduct of laying and molting hens, and its expression is changed in association with oviduct growth. Bangladesh J. Zool. 51(3): 345-359, 2023                                            
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