利用温度诱导响应技术鉴定耐高温大豆[Vigna mungo (L) Hepper]基因型

Vijay Laxmi, TN Tiwari, Rinki Devi
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引用次数: 0

摘要

植物的高温胁迫会降低作物产量,因为它会对植物的多个生理过程产生负面影响。在不断变化的环境中,筛选和鉴定适应高温胁迫条件的栽培品种至关重要。本研究采用温度诱导响应(TIR)技术筛选大豆(Vigna mungo (I) Hepper)的耐高温基因型。在本研究中,诱导温度和致死温度是根据恢复期结束时的生长减少百分比和存活百分比标准化的。诱导温度在 38 至 48 ºC 作为亚致死温度,即 4 小时和 30 分钟的挑战温度,而致死温度在 50 ºC 3 小时。共筛选和评估了 19 种大豆耐高温基因型。通过使用标准化的最适诱导温度和挑战温度,对所研究基因型的细胞耐受性进行了评估。根据诱导苗与对照苗相比的根长和芽长,计算出生长量减少最少和存活率最高的细胞水平耐受性,并确定 PGRU 95014 IPU 94-1、IPU 2K -22 和 PGRU 95016 为耐热基因型。这些基因型可作为育种计划的供体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of thermo tolerance urdbean [Vigna mungo (L) Hepper] genotypes using temperature induction response technique
High temperature stress in plants reduces crop yield because it negatively affects several plant physiological processes. Screening and identification of cultivars for high-temperature stress conditions is essential under changing environments. In the present study, the temperature induction response (TIR) technique was used for screening the high-temperature tolerant genotypes in urdbean (Vigna mungo (I) Hepper). In the present investigation, induction temperature and lethal temperature have been standardized based on percent growth reduction and survival percentage at the end of the recovery period. The induction temperature was observed at 38 to 48 ºC as sub-lethal i.e., challenging temperatures for 4 h and 30 min, while the lethal temperature was observed at 50 ºC for 3 h. This technique can be used as a potential tool to identify and select temperature-tolerant genotypes at the seedling stage from a large population. A total of nineteen urdbean genotypes were screened and evaluated for thermo tolerance. By using standardized optimum induction and challenging temperature, cellular level tolerance was assessed in the studied genotypes. Based on root length and shoot length of induced seedlings over control seedlings, the cellular level tolerance in terms of least reduction in growth and highest survival percentage was calculated and PGRU 95014 IPU 94-1, IPU 2K -22 and PGRU 95016 identified as heat tolerant genotypes. These can be used as donors in breeding programs.
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