色甘宁钠通过白细胞介素-6 水平调节卡拉胶诱发的急性炎症

IF 1 Q4 PHARMACOLOGY & PHARMACY
Ali Asghar Hemmati, M. Ghafourian, M. Khodayar, Hadi Zarin, Masoumeh Saberi Rounkian, Amir Jalali
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Thirty minutes after drug administration, carrageenan (1% w/v) was injected into the right hind paws of the rats. Changes in paw volume were measured using a plethysmometer at 0.5, 1, 2, and 3 hours post-carrageenan injection. Blood and paw tissue samples were collected three hours after carrageenan administration, and IL-6 levels were determined using the standard rat Interleukin-6 ELISA kit. Results: The plasma and tissue IL-6 levels in the group treated with cromolyn (25 mg/kg) were significantly higher than those in the positive control group (indomethacin 5 mg/kg). Sodium cromolyn at a dose of 25 mg/kg had a negligible effect on reducing paw edema and IL-6 levels in serum and paw tissues compared to indomethacin (5 mg/kg). The IL-6 levels in plasma and tissues for the group receiving 50 mg/kg of cromolyn were not significantly different from those in the indomethacin group (5 mg/kg). However, IL-6 levels in the group treated with 100 mg/kg of cromolyn were significantly lower than those in the indomethacin group. Among the groups treated with sodium cromolyn, the highest and lowest IL-6 levels were observed in the groups receiving 25 mg/kg and 100 mg/kg of sodium cromolyn, respectively. The anti-inflammatory effects of sodium cromolyn at doses of 25 mg/kg, 50 mg/kg, and 100 mg/kg were analyzed, revealing that 25 mg/kg of cromolyn sodium was not significantly effective at any time point (P < 0.05). Additionally, no significant differences (P < 0.05) were observed between the 50 mg/kg and 100 mg/kg doses at any study hour. Sodium cromolyn at a 50 mg/kg dose was as effective as indomethacin (5 mg/kg) in significantly reducing paw edema. Conclusions: Sodium cromolyn, at doses of 50 and 100 mg/kg, effectively reduced paw edema and the concentration of serum and paw tissue IL-6 (P < 0.05), demonstrating significant anti-inflammatory activity. 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引用次数: 0

摘要

背景:以往的研究表明,色甘酸钠不会对类固醇和非甾体抗炎药(NSAID)产生负面影响。研究目的本研究旨在探讨色甘酸钠对卡拉胶诱导的大鼠爪水肿的抗炎作用,并评估其对抗炎性细胞因子 IL-6 的影响。研究方法卡拉胶诱导的爪水肿模型被广泛用于研究炎症机制和评估抗炎药物。试验组腹腔注射 25、50 和 100 毫克/千克剂量的色甘酸钠,同时接受阳性对照组(吲哚美辛,5 毫克/千克)和阴性对照组(接受溶剂生理盐水)的治疗。给药 30 分钟后,向大鼠右后爪注射卡拉胶(1% w/v)。在注射卡拉胶后的 0.5、1、2 和 3 小时,使用褶压计测量爪子体积的变化。在注射卡拉胶三小时后收集血液和爪组织样本,并使用标准的大鼠白细胞介素-6 ELISA 试剂盒测定 IL-6 水平。结果接受色瑞林治疗组(25 毫克/千克)的血浆和组织 IL-6 水平明显高于阳性对照组(吲哚美辛 5 毫克/千克)。与吲哚美辛(5 毫克/千克)相比,25 毫克/千克剂量的色甘酸钠对减轻爪水肿以及降低血清和爪组织中 IL-6 水平的作用微乎其微。接受 50 毫克/千克色氨醇治疗组血浆和组织中的 IL-6 水平与吲哚美辛组(5 毫克/千克)没有显著差异。然而,接受 100 毫克/千克色甘酸钠治疗组的 IL-6 水平明显低于吲哚美辛组。在接受色瑞林钠治疗的各组中,接受 25 毫克/千克和 100 毫克/千克色瑞林钠治疗组的 IL-6 水平分别最高和最低。对 25 毫克/千克、50 毫克/千克和 100 毫克/千克剂量的色甘酸钠的抗炎效果进行分析后发现,25 毫克/千克的色甘酸钠在任何时间点都没有显著效果(P < 0.05)。此外,50 毫克/千克和 100 毫克/千克剂量在任何研究时间点均无明显差异(P < 0.05)。在显著减轻爪水肿方面,50 毫克/千克剂量的色甘酸钠与 5 毫克/千克剂量的吲哚美辛同样有效。结论剂量为 50 毫克/千克和 100 毫克/千克的色甘酸钠能有效减轻爪水肿,降低血清和爪组织 IL-6 的浓度(P < 0.05),显示出显著的抗炎活性。建议将 50 毫克/千克剂量的色甘酸钠作为该药物的抗炎剂量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sodium Cromolyn Modulates Carrageenan-Induced Acute Inflammation Through the Interleukin-6 Levels
Background: Previous studies have indicated that sodium cromolyn does not negatively affect steroidal and nonsteroidal anti-inflammatory drugs (NSAIDs). Objective: This study aimed to investigate the anti-inflammatory effects of sodium cromolyn on carrageenan-induced paw edema in rats and assess its impact on IL-6, an anti-inflammatory cytokine. Methods: The carrageenan-induced paw edema model is widely used for studying inflammatory mechanisms and evaluating anti-inflammatory drugs. The test groups received intraperitoneal injections of sodium cromolyn at doses of 25, 50, and 100 mg/kg alongside a positive control group (indomethacin at 5 mg/kg) and a negative control group (which received the solvent, saline). Thirty minutes after drug administration, carrageenan (1% w/v) was injected into the right hind paws of the rats. Changes in paw volume were measured using a plethysmometer at 0.5, 1, 2, and 3 hours post-carrageenan injection. Blood and paw tissue samples were collected three hours after carrageenan administration, and IL-6 levels were determined using the standard rat Interleukin-6 ELISA kit. Results: The plasma and tissue IL-6 levels in the group treated with cromolyn (25 mg/kg) were significantly higher than those in the positive control group (indomethacin 5 mg/kg). Sodium cromolyn at a dose of 25 mg/kg had a negligible effect on reducing paw edema and IL-6 levels in serum and paw tissues compared to indomethacin (5 mg/kg). The IL-6 levels in plasma and tissues for the group receiving 50 mg/kg of cromolyn were not significantly different from those in the indomethacin group (5 mg/kg). However, IL-6 levels in the group treated with 100 mg/kg of cromolyn were significantly lower than those in the indomethacin group. Among the groups treated with sodium cromolyn, the highest and lowest IL-6 levels were observed in the groups receiving 25 mg/kg and 100 mg/kg of sodium cromolyn, respectively. The anti-inflammatory effects of sodium cromolyn at doses of 25 mg/kg, 50 mg/kg, and 100 mg/kg were analyzed, revealing that 25 mg/kg of cromolyn sodium was not significantly effective at any time point (P < 0.05). Additionally, no significant differences (P < 0.05) were observed between the 50 mg/kg and 100 mg/kg doses at any study hour. Sodium cromolyn at a 50 mg/kg dose was as effective as indomethacin (5 mg/kg) in significantly reducing paw edema. Conclusions: Sodium cromolyn, at doses of 50 and 100 mg/kg, effectively reduced paw edema and the concentration of serum and paw tissue IL-6 (P < 0.05), demonstrating significant anti-inflammatory activity. Sodium cromolyn at a 50 mg/kg dose is recommended as the anti-inflammatory dose of this drug.
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CiteScore
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