传统饲养的巴厘牛的嗜酸性粒细胞和红细胞的组织形态测定法

Titi Humairah Bahtiar, N. K. Suwiti, I. K. Suatha
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引用次数: 0

摘要

血液组织是巴厘牛健康状况的一个指标。红细胞或红血球以及嗜酸性粒细胞等白细胞的存在非常重要,因为它们在感染过程中发挥着作用。因此,本研究旨在确定巴厘岛牛嗜酸性粒细胞和红细胞的组织学和形态学以及嗜酸性粒细胞的数量。所用样本为 30 头奶牛,取自布勒伦地区库布坦巴汉县布连村饲养的巴厘牛。血液组织经 MDT 染色制备而成。使用 EP View 应用程序以 1000 倍放大率对嗜酸性粒细胞和红细胞白血球进行形态测量。计算每 100 个白细胞中嗜酸性粒细胞的数量。结果显示,嗜酸性粒细胞呈圆形,有紫红色的二叶核和亮粉红色的颗粒状胞质,平均直径为 5.90 ± 1.04 µm。红细胞呈盘状,无紫色细胞核,有多种椭圆形细胞,平均直径为 3.62 ± 0.19 µm。传统饲养的巴厘牛的白细胞嗜酸性粒细胞平均为 7%。需要进一步开展研究,通过观察其他指标(如常规饲养巴厘牛的生理状态)来评估健康状况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
HISTOMORPHOMETRY OF EOSINOPHILS AND ERYTHROCYTES OF CONVENTIONALLY REARED BALI CATTLE
Blood tissue is an indicator of the health status of Balinese cattle. The presence of red blood cells or erythrocytes and white blood cells such as eosinophils is very important because they play a role in the infection process. Therefore, this study aims to determine the histology and morphometry of eosinophils and erythrocytes as well as the number of eosinophils in Balinese cattle raised in a conventional way. The samples used were 30 cows, taken from Balinese cows raised in Bulian Village, Kubutambahan District, Buleleng Regency. The blood tissue was prepared with MDT staining. Morphometric measurement of eosinophil and erythrocyte white blood cells using EP View application with 1000x magnification. Counting the number of eosinophils was done per 100 leukocyte cells. The results showed that eosinophils were round in shape and had a purplish-colored 2-lobed nucleus and bright pink granulated cytoplasm with an average diameter of 5.90 ± 1.04 µm. Erythrocytes have a discocyte shape without a purple nucleus and several variations of elliptocyte shapes with a mean diameter of 3.62 ± 0.19 µm. The mean white blood cell eosinophils of conventionally reared Balinese cattle was 7%. Further research is needed to assess health status by looking at other indicators such as physiological status in conventionally reared Balinese cattle.
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