原位和离位 Biofloc 对 Etroplus suratensis 的生长、免疫生理反应、营养成分和肌肉生长相关基因表达的影响

IF 0.4 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Indian Journal of Animal Research Pub Date : 2024-04-11 DOI:10.18805/ijar.b-5306

A. Jackqulinwino, B. Ahilan, Cheryl Antony, P. Chidambaram, A. Uma, P. Ruby

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引用次数: 0

摘要

背景：我们进行了一项为期 90 天的试验，研究原位和离位生物絮凝物对免疫生理反应、营养成分和肌肉生长相关基因表达的影响。实验方法实验组包括 IB-1、IB-2、IB-3（原位生物絮团），EB-1、EB-2、EB-3（异位生物絮团）。在容量为 2.4 吨的室外水箱中放养了 4.36±0.1 克珍珠斑（Etroplus suratensis）幼鱼，150 克/立方米。使用有机琼脂作为碳源，维持 12:1、15:1 和 20:1 的 C/N（IB-1、IB-2、IB-3）比，以促进原位生物絮团的发育和原位生物絮团的大量繁殖，并在日粮中添加 10%、20% 和 30%的分级饲料（EB-1、EB-2、EB-3）。结果实验结束时，与原位处理相比，原位生物絮团系统在 SOD、RBT、MPO、GLU、CAT 和血清蛋白方面的生理表现明显更好。在原位系统中，添加 20% 的生物絮凝物可明显改善营养成分。原位和离位生物絮凝物处理对全身氨基酸（如苯丙氨酸、亮氨酸、异亮氨酸、蛋氨酸、色氨酸和赖氨酸）有明显影响。然而，在 IB-3 原位处理和 EB-2 原位处理中，肌肉生长相关的 myo D 基因被显著上调，而在 IB-3 和 EB-3 中，肌生长蛋白被下调。因此，本研究建议采用原位生物絮凝法获得珍珠点的更佳表现，20:1 C: N 水平的原位生物絮凝可用于改善 Etroplus suratensis 的整体健康和肌肉生长。

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Efficacy of in situ and ex situ Biofloc on Growth, Immune-physiological Responses, Nutrient Composition and Muscle Growth-related Gene Expression of Etroplus suratensis

Background: A 90-day trial was carried out to investigate the effects of insitu and exsitu biofloc on the immune-physiological responses, nutrient composition and muscle growth-related gene expression. Methods: The experimental groups consisted of IB-1, IB-2, IB-3 (insitu biofloc), EB-1, EB-2, EB-3 (exsitu biofloc). Capacity of 2.4-ton outdoor tanks were stocked with 4.36±0.1 g pearlspot (Etroplus suratensis) juveniles @ 150/m3. Organic jaggery was used as carbon source to maintain 12:1, 15:1, and 20:1 C/N (IB-1, IB-2, IB-3) ratio for insitu biofloc development and exsitu biofloc mass production and feed supplementation at 10, 20 and 30 % graded level in the diet (EB-1, EB-2, EB-3). Result: At the end of the experiment, insitu biofloc system showed significantly better physiological performances in terms of SOD, RBT, MPO, GLU, CAT, and Serum protein compared to exsitu treatment. In exsitu system, biofloc supplemented at 20% significantly improved nutrient composition. There was a significant effect of insitu and exsitu biofloc treatments on whole-body amino acids like Phenylalanine, Leucine, Isoleucine, methionine, tryptophan, and lysine. However, the targeted muscle-growth-related myo D gene was significantly upregulated in IB-3 insitu and EB-2 exsitu treatments, while the myostatin was downregulated in IB-3 and EB-3. Therefore, this present study suggests the adoption of the insitu biofloc method to obtain better performance of Pearlspot, insitu biofloc at 20:1 C: N level could be used to improve overall health and muscle growth in Etroplus suratensis.

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来源期刊

Indian Journal of Animal Research AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.00

自引率

20.00%

发文量

332

审稿时长

6 months

期刊介绍： The IJAR, the flagship print journal of ARCC, it is a monthly journal published without any break since 1966. The overall aim of the journal is to promote the professional development of its readers, researchers and scientists around the world. Indian Journal of Animal Research is peer-reviewed journal and has gained recognition for its high standard in the academic world. It anatomy, nutrition, production, management, veterinary, fisheries, zoology etc. The objective of the journal is to provide a forum to the scientific community to publish their research findings and also to open new vistas for further research. The journal is being covered under international indexing and abstracting services.