确定贯叶连翘提取物对大鼠卵巢缺血和再灌注的保护作用

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
Seyit Ali Bingöl, S. Yiğit, Ergin Taşkın, F. Kacı, M. Yayla, N. Baygutalp, Erdem Toktay, Nilnur Eyerci, H. Çılgın
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引用次数: 0

摘要

目的:卵巢扭转是一种重要的妇科急症,因为它会导致卵巢缺血。我们的目的是研究金丝桃对缺血再灌注(I/R)损伤大鼠卵巢中 p53、TNF-α、CAT、SOD、GSH、PTEN 以及 haPI3K/Akt/mTOR 通路的影响。材料与方法:本研究将 56 只成年雌性大鼠分为 7 组:对照组、缺血组、缺血 300HP 组、缺血 600HP 组、I/R 组、I/R 300HP 组和 I/R 600HP 组。在缺血 300HP 组、缺血 600HP 组、I/R 300HP 组和 I/R 600HP 组中,以 300 mg/kg 或 600 mg/kg 的剂量口服穿心莲。采集的卵巢组织通过光学显微镜、生化和实时 PCR 技术进行检测。结果显示在组织学检查中,除对照组外,600HP 组的变性程度最低。TUNEL 结果显示,其他组的细胞凋亡水平低于缺血组和 I/R 组。I/R 600HP 组的 CAT 和 GSH 水平升高。缺血组的 PTEN 和 mTOR 水平最高。I/R 组和 I/R 300HP 组的 Akt 水平较低,但与对照组和 I/R 600HP 组接近。I/R 组的 TNF-α 和 p53 mRNA 表达增加。结论研究结果表明,穿心莲(600 mg/kg)对组织变性、CAT和GSH水平以及TNF-α和p53 mRNA表达水平均有影响。我们认为,穿心莲可作为缺血再灌注过程中保护组织免受损伤的一种药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of the Protective Impact of Hypericum perforatum Extract Against Ovarian Ischemia and Reperfusion in Rats
Objectives: Ovarian torsion is an important gynaecological emergency because it causes ischemia in ovary. Our aim is to research the effects of Hypericum perforatum on p53, TNF-α, CAT, SOD, GSH, PTEN and is haPI3K/Akt/mTOR pathway in ovarian of rats with ischemia reperfusion (I/R) injury. Materials and Methods: The study included 56 adult female rats which were allocated to 7 groups; control, ischemia, ischemia 300HP, ischemia 600HP, I/R, I/R 300HP and I/R 600HP. H. perforatum was applied to ischemia 300HP, ischemia 600HP, I/R 300HP and I/R 600HP groups at the dose of 300 mg/kg or 600 mg/kg by oral gavage. Collected ovarian tissues were examined by light microscopy, biochemical and real-time PCR techniques. Results: In histological examination, the least degeneration was seen in 600HP group among other groups, apart from control group. TUNEL results showed that apoptosis level of other groups was lower than that of ischemia and I/R groups. CAT and GSH levels of I/R 600HP group increased. The highest level of PTEN and mTOR was found in the ischemia group. The Akt level was lower in I/R and I/R 300HP groups but its level was close to that of control and I/R 600HP groups. TNF-α and p53 mRNA expressions in I/R group were increased. Conclusions: The result of the study showed that H. perforatum (600 mg/kg) had an effect on tissue degeneration, CAT and GSH levels, and also TNF-α and p53 mRNA expressions levels. We suggest that H. perforatum can be considered as an agent to protect against tissues damage during ischemia reperfusion.
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来源期刊
自引率
25.00%
发文量
15
审稿时长
8 weeks
期刊介绍: All kind of knowledge contributing to the development of science by its content, value, level and originality will be covered by CJMB. Problems of public health and their solutions are at the head of the windows opening us to the world. The "Crescent Journal of Medical and Biological Sciences" is a modern forum for scientific communication,coveringall aspects medical sciences and biological sciences, in basic and clinical sciences, mainly including: • Anatomy • Antioxidant Therapy in Reproduction Medicine • Biochemistry • Biophysics • Breast Cancer • Cardiology and Cardiovascular Medicine • Cell Biology • Dentistry sciences • Diabetes • Embryology • Endocrinology • Genetics • Hematology • Herbal Medicine • Histology • Internal Medicine • Internal Medicine, surgery • Medical Education • Medical Laboratory Sciences • Medical Microbiology • Microbiology • Mycology, Neurosciences • Nerosciences • Nutrition • Oncology • Parasitology • Pathology • Pharmacognosy • Pharmacology • Psychiatry • Sex-Based Biology • Sports Medicine • Urogynecology • Virology
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