Prmt7是间充质干细胞通过调节BMP信号分化成骨所必需的。

IF 2.9 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

BMB Reports Pub Date : 2024-04-15 DOI:10.5483/bmbrep.2023-0203

T. Vuong, Yan Zhang, June Kim, Young-Eun Leem, Jong-Sun Kang

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引用次数: 0

摘要

精氨酸甲基化由蛋白质精氨酸甲基转移酶（Prmts）催化，在各种生物过程中发挥着关键作用。然而，Prmts 在间充质干细胞（MSCs）成骨分化过程中的功能尚未得到明确了解。在本研究中，我们试图阐明Prmt7在成骨分化中的积极作用。与野生型细胞相比，去除了Prmt7的C3H/10T1/2细胞或骨髓间充质干细胞（BMSCs）的成骨特异基因表达和茜素红染色均有所减弱。此外，我们还发现，Prmt7 缺乏会降低骨形态发生蛋白（BMP）信号级联的激活，而BMP 信号级联对细胞命运承诺和成骨过程的调控至关重要。综上所述，我们的数据表明，Prmt7 在成骨分化过程中发挥着重要的调控作用。

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Prmt7 is required for the osteogenic differentiation of mesenchymal stem cells via modulation of BMP signaling.

Arginine methylation, which is catalyzed by protein arginine methyltransferases (Prmts), is known to play a key role in various biological processes. However, the function of Prmts in osteogenic differentiation of mesenchymal stem cells (MSCs) has not been clearly understood. In the current study, we attempted to elucidate a positive role of Prmt7 in osteogenic differentiation. Prmt7-depleted C3H/10T1/2 cells or bone marrow mesenchymal stem cells (BMSCs) showed the attenuated expression of osteogenic specific genes and Alizarin red staining compared to the wild-type cells. Furthermore, we found that Prmt7 deficiency reduced the activation of bone morphogenetic protein (BMP) signaling cascade, which is essential for the regulation of cell fate commitment and osteogenesis. Taken together, our data indicate that Prmt7 plays important regulatory roles in osteogenic differentiation.

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来源期刊

BMB Reports 生物-生化与分子生物学

CiteScore

5.10

自引率

7.90%

发文量

141

审稿时长

1 months

期刊介绍： The BMB Reports (BMB Rep, established in 1968) is published at the end of every month by Korean Society for Biochemistry and Molecular Biology. Copyright is reserved by the Society. The journal publishes short articles and mini reviews. We expect that the BMB Reports will deliver the new scientific findings and knowledge to our readers in fast and timely manner.