{"title":"损伤大鼠坐骨神经细胞中的 connexin-43","authors":"E. Kolos","doi":"10.17816/morph.629037","DOIUrl":null,"url":null,"abstract":"AIM: The purpose of this study was to study the distribution and localization of the gap junction protein connexin-43 (Cx43) in cells of intact and injured rat sciatic nerve. \nMETHODS: Damage to the sciatic nerves of Wistar rats of the experimental group (n=5) was carried out by nerve ligation for 40 s. Animals without damage of sciatic nerve were studied as a control group (n=5). Immunohistochemical detection of connexin-43 was performed on paraffin sections. \nRESULTS: It has been established that the protein is contained in the cells of the perineurium and epineurium of both the intact nerve and after the application of a ligature. In the area of the endoneurium, in the absence of nerve damage, Cx43 is detected only in the endotheliocytes of a few vessels. In the endoneurium of the injured nerve, a large number of large Cx43-immunopositive cells with processes were identified. \nCONCLUSION: It has been established that Cx43-containing cells are identified in the endoneurium of the sciatic nerve only after damage.","PeriodicalId":506132,"journal":{"name":"Morphology","volume":"158 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CONNEXIN-43 IN CELLS OF INJURED RAT SCIATIC NERVE\",\"authors\":\"E. Kolos\",\"doi\":\"10.17816/morph.629037\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"AIM: The purpose of this study was to study the distribution and localization of the gap junction protein connexin-43 (Cx43) in cells of intact and injured rat sciatic nerve. \\nMETHODS: Damage to the sciatic nerves of Wistar rats of the experimental group (n=5) was carried out by nerve ligation for 40 s. Animals without damage of sciatic nerve were studied as a control group (n=5). Immunohistochemical detection of connexin-43 was performed on paraffin sections. \\nRESULTS: It has been established that the protein is contained in the cells of the perineurium and epineurium of both the intact nerve and after the application of a ligature. In the area of the endoneurium, in the absence of nerve damage, Cx43 is detected only in the endotheliocytes of a few vessels. In the endoneurium of the injured nerve, a large number of large Cx43-immunopositive cells with processes were identified. \\nCONCLUSION: It has been established that Cx43-containing cells are identified in the endoneurium of the sciatic nerve only after damage.\",\"PeriodicalId\":506132,\"journal\":{\"name\":\"Morphology\",\"volume\":\"158 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Morphology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17816/morph.629037\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Morphology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17816/morph.629037","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
AIM: The purpose of this study was to study the distribution and localization of the gap junction protein connexin-43 (Cx43) in cells of intact and injured rat sciatic nerve.
METHODS: Damage to the sciatic nerves of Wistar rats of the experimental group (n=5) was carried out by nerve ligation for 40 s. Animals without damage of sciatic nerve were studied as a control group (n=5). Immunohistochemical detection of connexin-43 was performed on paraffin sections.
RESULTS: It has been established that the protein is contained in the cells of the perineurium and epineurium of both the intact nerve and after the application of a ligature. In the area of the endoneurium, in the absence of nerve damage, Cx43 is detected only in the endotheliocytes of a few vessels. In the endoneurium of the injured nerve, a large number of large Cx43-immunopositive cells with processes were identified.
CONCLUSION: It has been established that Cx43-containing cells are identified in the endoneurium of the sciatic nerve only after damage.
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