TaNAM-A1自然变异在控制小麦籽粒灌浆期叶片衰老方面的功能差异。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Longxi Zhou, Guowei Chang, Chuncai Shen, Wan Teng, Xue He, Xueqiang Zhao, Yanfu Jing, Zhixiong Huang, Yiping Tong
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引用次数: 0

摘要

叶片衰老是与谷物产量潜力和营养品质相关的重要生理过程。花后绿叶持续时间(GLD)直接反映了叶片衰老过程,在普通小麦中表现出较大的基因型差异;然而,其背后的基因调控机制仍然缺乏。在此,我们通过基于图谱的克隆鉴定出 TaNAM-A1 是谷粒灌浆期 GLD 的主要位点 qGLD-6A 的因果基因。转基因试验和TILLING突变体分析表明,TaNAM-A1在调控叶片衰老中起关键作用,同时也影响穗长和籽粒大小。此外,还系统评估了 TaNAM-A1 三个单倍型之间的功能差异。与野生型 TaNAM-A1a 相比,TaNAM-A1d(包含 TaNAM-A1 编码 DNA 序列中的两个突变)的小麦品种表现出更长的 GLD 和更优越的产量相关性状。所有三种单倍型都能激活参与大分子降解和矿质养分再动员的基因的表达,其中 TaNAM-A1a 的活性最强,TaNAM-A1d 的活性最弱。TaNAM-A1 还能调节衰老相关转录因子 TaNAC-S-7A 和 TaNAC016-3A 的表达。TaNAC016-3A通过蛋白-蛋白相互作用增强了TaNAM-A1a的转录激活能力,从而促进了衰老过程。我们的研究为不同地理气候条件下小麦育种提供了叶片功能期和谷粒产量形成微调的新见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Functional divergences of natural variations of TaNAM-A1 in controlling leaf senescence during wheat grain filling

Functional divergences of natural variations of TaNAM-A1 in controlling leaf senescence during wheat grain filling

Leaf senescence is an essential physiological process related to grain yield potential and nutritional quality. Green leaf duration (GLD) after anthesis directly reflects the leaf senescence process and exhibits large genotypic differences in common wheat; however, the underlying gene regulatory mechanism is still lacking. Here, we identified TaNAM-A1 as the causal gene of the major loci qGLD-6A for GLD during grain filling by map-based cloning. Transgenic assays and TILLING mutant analyses demonstrated that TaNAM-A1 played a critical role in regulating leaf senescence, and also affected spike length and grain size. Furthermore, the functional divergences among the three haplotypes of TaNAM-A1 were systematically evaluated. Wheat varieties with TaNAM-A1d (containing two mutations in the coding DNA sequence of TaNAM-A1) exhibited a longer GLD and superior yield-related traits compared to those with the wild type TaNAM-A1a. All three haplotypes were functional in activating the expression of genes involved in macromolecule degradation and mineral nutrient remobilization, with TaNAM-A1a showing the strongest activity and TaNAM-A1d the weakest. TaNAM-A1 also modulated the expression of the senescence-related transcription factors TaNAC-S-7A and TaNAC016-3A. TaNAC016-3A enhanced the transcriptional activation ability of TaNAM-A1a by protein–protein interaction, thereby promoting the senescence process. Our study offers new insights into the fine-tuning of the leaf functional period and grain yield formation for wheat breeding under various geographical climatic conditions.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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