环丙酚通过调节抑郁样大鼠海马的有氧糖酵解,改善 ECS 诱导的学习和记忆损伤

IF 3.3 3区 心理学 Q1 BEHAVIORAL SCIENCES
You Yang , Dongyu Zhou , Su Min , Di Liu , Mou Zou , Chang Yu , Lihao Chen , Jia Huang , Ruiyang Hong
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引用次数: 0

摘要

电休克(ECS)可用于治疗抑郁症,但可能会导致学习/记忆障碍,而麻醉剂可通过调节海马突触可塑性来改善这种障碍。鉴于突触可塑性受有氧糖酵解的支配,目前仍不清楚麻醉剂是否会调节有氧糖酵解以增强学习和记忆功能。慢性轻度不可预知应激(CUMS)诱导大鼠出现抑郁样行为,并通过蔗糖偏好试验(SPT)评估失神。通过强迫游泳试验(FST)、开阔地试验(OFT)和莫里斯水迷宫试验(MWM)评估抑郁样行为和空间学习/记忆。使用免疫荧光分析、Western 印迹、乳酸检测试剂盒和透射电子显微镜记录了ECS后抑郁样大鼠海马区有氧糖酵解和突触可塑性的变化。OFT和FST均表明,ECS能有效缓解抑郁样行为。MWM测试表明,麻醉剂能够减轻ECS引起的学习和记忆障碍。免疫荧光分析、Western印迹、乳酸测定试剂盒和透射电子显微镜显示,ECS诱导的抑郁样大鼠学习和记忆能力的下降与有氧糖酵解减少有关,而额外使用环丙酚或异丙酚可改善这些改变。添加糖酵解抑制剂 2-DG 会减弱麻醉剂的改善作用。在增强ECS后星形胶质细胞的有氧糖酵解和突触可塑性方面,观察到环丙酚和异丙酚之间没有明显差异。这些发现可能有助于理解麻醉药物调节抑郁样行为大鼠ECS后学习和记忆损伤的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Ciprofol ameliorates ECS-induced learning and memory impairment by modulating aerobic glycolysis in the hippocampus of depressive-like rats

Ciprofol ameliorates ECS-induced learning and memory impairment by modulating aerobic glycolysis in the hippocampus of depressive-like rats

Electroconvulsive shock (ECS) is utilized to treat depression but may cause learning/memory impairments, which may be ameliorated by anesthetics through the modulation of hippocampal synaptic plasticity. Given that synaptic plasticity is governed by aerobic glycolysis, it remains unclear whether anesthetics modulate aerobic glycolysis to enhance learning and memory function. Depression-like behavior in rats was induced by chronic mild unpredictable stress (CUMS), with anhedonia assessed via sucrose preference test (SPT). Depressive-like behaviors and spatial learning/memory were assessed with forced swim test (FST), open field test (OFT), and Morris water maze (MWM) test. Changes in aerobic glycolysis and synaptic plasticity in the hippocampal region of depressive-like rats post-ECS were documented using immunofluorescence analysis, Western blot, Lactate Assay Kit and transmission electron microscopy. Both the OFT and FST indicated that ECS was effective in alleviating depressive-like behaviors. The MWM test demonstrated that anesthetics were capable of attenuating ECS-induced learning and memory deficits. Immunofluorescence analysis, Western blot, Lactate Assay Kit and transmission electron microscopy revealed that the decline in learning and memory abilities in ECS-induced depressive-like rats was correlated with decreased aerobic glycolysis, and that the additional use of ciprofol or propofol ameliorated these alterations. Adding the glycolysis inhibitor 2-DG diminished the ameliorative effects of the anesthetic. No significant difference was observed between ciprofol and propofol in enhancing aerobic glycolysis in astrocytes and synaptic plasticity after ECS. These findings may contribute to understanding the mechanisms by which anesthetic drugs modulate learning and memory impairment after ECS in depressive-like behavior rats.

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来源期刊
CiteScore
6.40
自引率
2.80%
发文量
122
审稿时长
38 days
期刊介绍: Pharmacology Biochemistry & Behavior publishes original reports in the areas of pharmacology and biochemistry in which the primary emphasis and theoretical context are behavioral. Contributions may involve clinical, preclinical, or basic research. Purely biochemical or toxicology studies will not be published. Papers describing the behavioral effects of novel drugs in models of psychiatric, neurological and cognitive disorders, and central pain must include a positive control unless the paper is on a disease where such a drug is not available yet. Papers focusing on physiological processes (e.g., peripheral pain mechanisms, body temperature regulation, seizure activity) are not accepted as we would like to retain the focus of Pharmacology Biochemistry & Behavior on behavior and its interaction with the biochemistry and neurochemistry of the central nervous system. Papers describing the effects of plant materials are generally not considered, unless the active ingredients are studied, the extraction method is well described, the doses tested are known, and clear and definite experimental evidence on the mechanism of action of the active ingredients is provided.
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