用 SPRi 测定子宫内膜异位症妇女血浆和腹腔液中的酪蛋白酶 L 和 S

IF 2.5 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Advances in medical sciences Pub Date : 2024-04-18 DOI:10.1016/j.advms.2024.04.004

Julia Załęcka , Zuzanna Zielińska , Łukasz Ołdak , Agata Sakowicz , Grzegorz Mańka , Mariusz Kiecka , Robert Spaczyński , Piotr Piekarski , Beata Banaszewska , Artur Jakimiuk , Tadeusz Issat , Jakub Młodawski , Maria Szubert , Piotr Sieroszewski , Grzegorz Raba , Kamil Szczupak , Tomasz Kluz , Marek Kluza , Piotr Pierzyński , Michał Ciebiera , Piotr Laudański

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引用次数: 0

摘要

目的子宫内膜异位症是一种常见疾病，病理机制复杂，症状不典型，往往导致诊断延误。目前，确诊该病的唯一方法是通过腹腔镜检查和对采集的组织进行组织病理学检查。然而，这种侵入性手术存在潜在风险和并发症，因此有必要探索子宫内膜异位症的非手术诊断方法。本研究旨在分析腹腔液和血浆样本中猫胰蛋白酶 L 和猫胰蛋白酶 S 的表达，以确定子宫内膜异位症非侵入性诊断方法的潜在生物标志物。研究组由确诊为子宫内膜异位症的妇女组成。研究结果显示，对照组和研究组腹腔液和血浆中的钙蛋白 L 和钙蛋白 S 的浓度没有明显差异。

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The SPRi determination of cathepsin L and S in plasma and peritoneal fluid of women with endometriosis

Purpose

Endometriosis is a common disease with a complex pathomechanism and atypical symptoms, often leading to delayed diagnosis. Currently, the sole method for confirming the presence of the disease is through laparoscopy and histopathological examination of collected tissue. However, this invasive procedure carries potential risk and complications, necessitating the exploration of non-surgical diagnostic methods for endometriosis. This study aims to analyze peritoneal fluid and plasma samples for the expression of cathepsin L and cathepsin S to identify potential biomarkers for non-invasive diagnostic approaches to endometriosis.

Material and methods

In this cross-sectional study, plasma and peritoneal fluid samples were obtained during laparoscopy from 63 patients diagnosed with chronic pelvic pain or infertility. The study group consisted of women with confirmed endometriosis. The concentrations of cathepsins L and S were determined using an SPRi biosensor.

Results

The study did not reveal significant differences in the concentrations of cathepsin L and cathepsin S between the control group and the study group, both in peritoneal fluid and plasma.

Conclusions

Based on the results of this study, it appears that cathepsins L and S are not suitable candidates as biomarkers for endometriosis.

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来源期刊

Advances in medical sciences 医学-医学：研究与实验

CiteScore

5.00

自引率

0.00%

发文量

审稿时长

25 days

期刊介绍： Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.