Harald W. Platta, Julia Jeske, Nadine Schmidt, Ralf Erdmann
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引用次数: 0
摘要
过氧物酶体是一种细胞器,在脂质代谢和细胞氧化还原平衡中发挥着核心作用。过氧化物酶体靶向信号(PTS)受体导入过氧化物酶体基质蛋白是一种 ATP 依赖性机制。然而,能量依赖性步骤不是在受体-货物复合体与膜结合的早期发生,而是在后期发生,因为它们与过氧化物酶体导出复合体相关联,以释放卸载的受体。第一个需要 ATP 的步骤是 PTS 受体的半胱氨酸依赖性单泛素化,这是 AAA+ 过氧化物酶识别所必需的。它们执行的第二个 ATP 依赖性步骤是将泛素化的 PTS 受体从膜中提取出来,释放回细胞质。去泛素化后,PTS 受体恢复了导入能力,并能促进下一轮的货物导入。在此,我们将对过氧化物酶体蛋白质导入过程中的 ATP 依赖性步骤进行概述并讨论最新数据。
Peroxisomes are organelles that play a central role in lipid metabolism and cellular redox homeostasis. The import of peroxisomal matrix proteins by peroxisomal targeting signal (PTS) receptors is an ATP-dependent mechanism. However, the energy-dependent steps do not occur early during the binding of the receptor–cargo complex to the membrane but late, because they are linked to the peroxisomal export complex for the release of the unloaded receptor. The first ATP-demanding step is the cysteine-dependent monoubiquitination of the PTS receptors, which is required for recognition by the AAA+ peroxins. They execute the second ATP-dependent step by extracting the ubiqitinated PTS receptors from the membrane for release back to the cytosol. After deubiquitination, the PTS receptors regain import competence and can facilitate further rounds of cargo import. Here, we give a general overview and discuss recent data regarding the ATP-dependent steps in peroxisome protein import.
期刊介绍:
The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.