Fan Huang, Chuan Ming, Yuqian Jiang, Chenli Li, Cheng Tan
{"title":"构建和验证急性髓性白血病儿童关键基因相关预后模型","authors":"Fan Huang, Chuan Ming, Yuqian Jiang, Chenli Li, Cheng Tan","doi":"10.1111/ijlh.14272","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>To identify the differentially expressed genes of acute myeloid leukaemia (AML) and construct and verify a survival prognosis model combined with patient survival information.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>The TARGET database was searched to identify differentially expressed peripheral blood genes in children with AML and healthy children. A gene set functional analysis and pathway analysis were performed using gene ontology and the KEGG pathway. A prognostic model for children with AML was constructed using univariate Cox, LASSO Cox regression and multivariate Cox regression analyses. Time-dependent receiver operating characteristic (ROC) curves were adopted to assess the predictive capacity of the prognostic models.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In total, 1640 differentially expressed genes were screened (1119 upregulated and 521 downregulated genes). The differentially expressed genes were mainly involved in nutrient metabolism and cytochrome P450 metabolism. Six key genes related to the prognosis of AML, <i>FAM157A</i>, <i>GPR78</i>, <i>IRX5</i>, <i>RP4-800G7.1</i>, <i>RP11-179H18.5</i> and <i>RP11-61N20.3</i>, were identified. Kaplan–Meier curves indicated that 3-year and 5-year overall survival was significantly higher in the low-risk group than in the high-risk group. The area under the ROC curve was 0.722. At different stages of AML, <i>FAM157A</i> and <i>RP4-800G7.1</i> exhibited significant differences in expression. The expression levels of <i>FAM157A</i> were significantly decreased in AML, whereas the expression levels of <i>GPR78</i>, <i>IRX5</i>, <i>RP4-800G7.1</i>, <i>RP11-179H18.5</i> and <i>RP11-61N20.3</i> were significantly increased in AML.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>A prognosis-related gene model of AML was successfully constructed, and the expression levels of the model genes varied with AML stage.</p>\n </section>\n </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 4","pages":"678-686"},"PeriodicalIF":2.2000,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Construction and validation of key genes-related prognosis model in children with acute myeloid leukaemia\",\"authors\":\"Fan Huang, Chuan Ming, Yuqian Jiang, Chenli Li, Cheng Tan\",\"doi\":\"10.1111/ijlh.14272\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Introduction</h3>\\n \\n <p>To identify the differentially expressed genes of acute myeloid leukaemia (AML) and construct and verify a survival prognosis model combined with patient survival information.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>The TARGET database was searched to identify differentially expressed peripheral blood genes in children with AML and healthy children. A gene set functional analysis and pathway analysis were performed using gene ontology and the KEGG pathway. A prognostic model for children with AML was constructed using univariate Cox, LASSO Cox regression and multivariate Cox regression analyses. Time-dependent receiver operating characteristic (ROC) curves were adopted to assess the predictive capacity of the prognostic models.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>In total, 1640 differentially expressed genes were screened (1119 upregulated and 521 downregulated genes). The differentially expressed genes were mainly involved in nutrient metabolism and cytochrome P450 metabolism. Six key genes related to the prognosis of AML, <i>FAM157A</i>, <i>GPR78</i>, <i>IRX5</i>, <i>RP4-800G7.1</i>, <i>RP11-179H18.5</i> and <i>RP11-61N20.3</i>, were identified. Kaplan–Meier curves indicated that 3-year and 5-year overall survival was significantly higher in the low-risk group than in the high-risk group. The area under the ROC curve was 0.722. At different stages of AML, <i>FAM157A</i> and <i>RP4-800G7.1</i> exhibited significant differences in expression. 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Construction and validation of key genes-related prognosis model in children with acute myeloid leukaemia
Introduction
To identify the differentially expressed genes of acute myeloid leukaemia (AML) and construct and verify a survival prognosis model combined with patient survival information.
Methods
The TARGET database was searched to identify differentially expressed peripheral blood genes in children with AML and healthy children. A gene set functional analysis and pathway analysis were performed using gene ontology and the KEGG pathway. A prognostic model for children with AML was constructed using univariate Cox, LASSO Cox regression and multivariate Cox regression analyses. Time-dependent receiver operating characteristic (ROC) curves were adopted to assess the predictive capacity of the prognostic models.
Results
In total, 1640 differentially expressed genes were screened (1119 upregulated and 521 downregulated genes). The differentially expressed genes were mainly involved in nutrient metabolism and cytochrome P450 metabolism. Six key genes related to the prognosis of AML, FAM157A, GPR78, IRX5, RP4-800G7.1, RP11-179H18.5 and RP11-61N20.3, were identified. Kaplan–Meier curves indicated that 3-year and 5-year overall survival was significantly higher in the low-risk group than in the high-risk group. The area under the ROC curve was 0.722. At different stages of AML, FAM157A and RP4-800G7.1 exhibited significant differences in expression. The expression levels of FAM157A were significantly decreased in AML, whereas the expression levels of GPR78, IRX5, RP4-800G7.1, RP11-179H18.5 and RP11-61N20.3 were significantly increased in AML.
Conclusion
A prognosis-related gene model of AML was successfully constructed, and the expression levels of the model genes varied with AML stage.
期刊介绍：
The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology.
The journal publishes invited reviews, full length original articles, and correspondence.
The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines.
The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.
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