苔藓和拟南芥锌指同源转录因子在调控植物结构中的作用

IF 1.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Young Koung Lee, Keunhwa Kim, Doreen Ware
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引用次数: 0

摘要

锌指同源基因转录因子(ZF-HD TFs)是拟南芥中一个相对较小的基因家族,参与植物的发育和胁迫响应。然而,ZF-HD TFs 的生物学功能在很大程度上仍未被发现。在此,我们通过对拟南芥、高粱(Sorghum bicolor)和苔藓(Physcomitrella patens)进行系统发育分析、结构域和基团鉴定研究,旨在阐明 ZF-HD TFs 在其他物种中的进化历史和功能作用。根据ZF富含Cys/His的二聚化结构域和同源结构域(HD),42个ZF-HD TF蛋白被分为两个不同的亚家族。根据序列的相似性,蛋白质的系统发生树被进一步分为五组,并在氨基酸序列中定义了三个不同的主题。遗传分析表明,苔藓 PpZF-HD1(Pp3c1_15290)基因在表型和分子水平上部分挽救了 amiR zf-HD-79 突变株系。亚细胞定位研究表明,苔藓 PpZF-HD1 定位于细胞质和细胞核中。系统发育分析和遗传互补揭示了ZF-HD TFs在调控植物结构中的功能作用,这在拟南芥、高粱和苔藓中是保守的。虽然我们的研究只是对ZF-HD TFs的初步探索,但它提供了一个新的视角,有助于未来的研究人员更好地理解ZF-HD蛋白在植物中的生物学作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of moss and Arabidopsis zinc-finger homeodomain transcription factors in regulating plant architecture

Zinc-finger homeodomain transcription factors (ZF-HD TFs) are relatively a small gene family in Arabidopsis involved in plant development and stress response. However, the biological functions of ZF-HD TFs remain largely undiscovered. Here, we aimed to elucidate the evolutionary history and functional role of ZF-HD TFs in other species, by performing phylogenic analysis and domain and motif identification studies in Arabidopsis, sorghum (Sorghum bicolor), and moss (Physcomitrella patens). Forty-two ZF-HD TF proteins were classified into two distinct subfamilies based on the conserved ZF Cys/His-rich dimerization and homeodomain (HD) domains. The phylogenetic tree of proteins was further divided into five groups based on the similarity of sequences, and three distinct motifs were defined in the amino acid sequences. Genetic analysis revealed that the moss PpZF-HD1, Pp3c1_15290, gene partially rescued the amiR zf-HD-79 mutant lines at phenotypic and molecular levels. Subcellular localization studies revealed that moss PpZF-HD1 was localized in the cytosol and nuclei. Phylogenetic analysis and genetic complementation revealed that ZF-HD TFs play functional roles in regulating plant architecture, which is conserved in Arabidopsis, sorghum, and moss. Although our study is only a preliminary exploration into ZF-HD TFs, it provides a novel perspective that will help future researchers better understand the biological role of ZF-HD proteins in plants.

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来源期刊
Plant Biotechnology Reports
Plant Biotechnology Reports 生物-生物工程与应用微生物
CiteScore
4.10
自引率
4.20%
发文量
72
审稿时长
>12 weeks
期刊介绍: Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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