棘尾蚴中的中性脂质、包囊元蚴和红蚴中的中性脂质

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-12 DOI:10.1017/s0022149x00701167

C.J. Marsit, B. Fried, J. Sherma

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引用次数: 0

摘要

采用高效薄层色谱法（HPTLC）分析了来自Biomphalaria glabrata蜗牛的Echinostoma caproni的红iae、carcariae和包囊元carcariae中的中性脂质。对色谱图的肉眼观察显示，所有阶段中最丰富的脂质部分都是游离固醇。游离固醇的定量显示，每条红虫的平均重量为 2.7 ± 0.64 纳克，每条螯虫的平均重量为 0.53 ± 0.023 纳克，每条包囊元螯虫的平均重量为 0.081 ± 0.0098 纳克。幼虫阶段的油红 O 染色证实了红虫和恙虫体内存在脂质，但包囊的元蛔虫体内没有脂质。从蛛网膜到包囊元蛛网膜阶段中性脂质的减少并不支持之前的观察结果，即脂肪在地衣虫生命周期的各个阶段都会增加。

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Neutral lipids in cercariae, encysted metacercariae, and rediae of Echinostoma caproni

High performance thin-layer chromatography (HPTLC) was used to analyse the neutral lipids in the rediae, cercariae, and encysted metacercariae of Echinostoma caproni from Biomphalaria glabrata snails. Visual observations of the chromatograms showed that the most abundant lipid fraction in all stages was free sterol. Quantification of the free sterol revealed mean weights of 2.7 ± 0.64 ng per redia, 0.53 ± 0.023 ng per cercaria, and 0.081 ± 0.0098 ng per encysted metacercaria. Oil Red O staining of the larval stages confirmed the presence of lipids within the rediae and cercariae but did not show lipids in the encysted metacercariae. The dimunition in neutral lipids from the cercarial to the encysted metacercarial stage does not support a previous observation that fat increases in successive phases of the digenean life cycle.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.