利用超高效液相色谱-三重四极杆质谱法快速分析鞘氨醇中的七种多胺

IF 2.3 3区化学 Q3 CHEMISTRY, ANALYTICAL

Journal of Analytical Methods in Chemistry Pub Date : 2024-04-16 DOI:10.1155/2024/3302455

Mingwen Zhang, Yunqiang Zhao, Zongwen Wang, Jintian Cheng

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引用次数: 0

摘要

基于超高效液相色谱-三重四极杆质谱（UPLC-3Q-MS）和液相萃取技术，建立了一种快速、简便、灵敏的同时测定鞘翅目昆虫体内七种多胺的方法。昆虫样品经 HClO4 溶液提取后，采用 UPLC-3Q-MS 进行分离和检测。该方法成功地检测了健康和二氟甲基鸟氨酸处理过的Nephotettix cincticeps成虫体内的7种多胺，方法检出限和方法定量限分别为24-139 pg/mg和82-464 pg/mg，日内和日间相对标准偏差（RSD，n = 5）分别为1.97-6.00%和2.08-5.92%，回收率为86-115%。这项研究的成功为快速、高通量检测昆虫样品中的多胺提供了一种可靠的方法。

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Rapid Analysis of Seven Polyamines in Nephotettix cincticeps by Using Ultra-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry

A fast, simple, and sensitive method for the simultaneous determination of seven polyamines in Nephotettix cincticeps was developed based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-3Q-MS) together with liquid phase extraction. Polyamines in insect samples were extracted with HClO₄ solution and then were separated and detected by UPLC-3Q-MS, which was equipped with a hydrophilic interaction liquid chromatography column, within 5 min without any derivatization procedure. The method has been successfully used to detect 7 polyamines in healthy and difluormethylornithine-treated adults of Nephotettix cincticeps with a method limit of detection and the method limit of quantitation of 24–139 pg/mg and 82–464 pg/mg, respectively, an intraday and interday relative standard deviation (RSD, n = 5) of 1.97–6.00% and 2.08–5.92% respectively, and a recovery of 86–115%. The success of this study provided a reliable method for the rapid and high-throughput detection of polyamines in the insect sample.

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来源期刊

Journal of Analytical Methods in Chemistry CHEMISTRY, ANALYTICAL-ENGINEERING, CIVIL

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical. Subject areas include (but are by no means limited to): Separation Spectroscopy Mass spectrometry Chromatography Analytical Sample Preparation Electrochemical analysis Hyphenated techniques Data processing As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.