赭曲霉丝氨酸蛋白酶 PAPC 对革兰氏阳性和革兰氏阴性菌生物膜的破坏作用

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-01 DOI:10.1134/s0026261723604281

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引用次数: 0

摘要

摘要与革兰氏阳性和革兰氏阴性微生物形成的生物膜有关的感染给治疗带来困难，并容易转变为慢性感染。因此，我们需要降解生物膜基质的方法。在本研究中，我们发现来自赭曲霉的重组 PAPC 丝氨酸蛋白酶在 50 µg/mL 的浓度下能降解一些革兰氏阳性和革兰氏阴性细菌形成的成熟生物膜 15-20%。当浓度为 100 µg/mL 时，金黄色葡萄球菌和绿脓杆菌生物膜的生物量减少 50%。因此，PAPC 可能是一种很有前景的生物膜清除剂，并能提高抗菌治疗的效率。

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Destruction of Biofilms of Gram-Positive and Gram-Negative Bacteria by Serine Protease PAPC from Aspergillus ochraceus

Abstract

Infections associated with biofilm formation by gram-positive and gram-negative microorganisms cause difficulty in therapy and are prone to transition into chronic forms. Approaches to degradation of the biofilm matrix are therefore in demand. In the present work, we show that recombinant PAPC serine protease from Aspergillus ochraceus were able to degrade mature biofilms formed by a number of gram-positive and gram-negative bacteria by 15‒20% at 50 µg/mL. At 100 µg/mL, the biomass of S. aureus and P. aeruginosa biofilms decreased by 50%. Thus, the PAPC may be a promising agent for biofilm removal and enhance the efficiency of antimicrobial therapy.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.