单细胞分析揭示了 HNSCC 中以 B 细胞为主的免疫亚型,有助于加强预后和治疗分层

IF 10.8 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Kang Li, Caihua Zhang, Ruoxing Zhou, Maosheng Cheng, Rongsong Ling, Gan Xiong, Jieyi Ma, Yan Zhu, Shuang Chen, Jie Chen, Demeng Chen, Liang Peng
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引用次数: 0

摘要

头颈部鳞状细胞癌(HNSCC)的特点是高复发率或远处转移率,预后极具挑战性。越来越多的证据表明,肿瘤浸润B细胞(TIL-Bs)在肿瘤控制中发挥着至关重要的协同作用。然而,人们对TIL-Bs在免疫微环境中的作用以及TIL-Bs如何影响免疫检查点阻断的结果知之甚少。该研究利用基因表达总库(GEO)数据库中的单细胞RNA测序(scRNA-seq)数据,确定了TIL-Bs中不同的基因表达模式。利用无监督聚类将HNSCC样本分为TIL-Bs抑制组和TIL-Bs激活组。我们利用 TCGA HNSCC 数据进一步验证了这种分类方法,它与患者的预后、免疫细胞浸润和对免疫疗法的反应相关。我们发现,B 细胞激活组的预后较好,免疫细胞浸润较高,免疫检查点水平明显,包括 PD-L1 升高。我们还建立并验证了一个预后模型,其中强调了四个基因作为预测 HNSCC 患者生存结果的潜在生物标志物。总之,这项研究为基于 B 细胞的 HNSCC 肿瘤分类提供了一种基础方法,为靶向治疗和免疫疗法策略提供了启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Single cell analysis unveils B cell-dominated immune subtypes in HNSCC for enhanced prognostic and therapeutic stratification

Single cell analysis unveils B cell-dominated immune subtypes in HNSCC for enhanced prognostic and therapeutic stratification

Head and neck squamous cell carcinoma (HNSCC) is characterized by high recurrence or distant metastases rate and the prognosis is challenging. There is mounting evidence that tumor-infiltrating B cells (TIL-Bs) have a crucial, synergistic role in tumor control. However, little is known about the role TIL-Bs play in immune microenvironment and the way TIL-Bs affect the outcome of immune checkpoint blockade. Using single-cell RNA sequencing (scRNA-seq) data from the Gene Expression Omnibus (GEO) database, the study identified distinct gene expression patterns in TIL-Bs. HNSCC samples were categorized into TIL-Bs inhibition and TIL-Bs activation groups using unsupervised clustering. This classification was further validated with TCGA HNSCC data, correlating with patient prognosis, immune cell infiltration, and response to immunotherapy. We found that the B cells activation group exhibited a better prognosis, higher immune cell infiltration, and distinct immune checkpoint levels, including elevated PD-L1. A prognostic model was also developed and validated, highlighting four genes as potential biomarkers for predicting survival outcomes in HNSCC patients. Overall, this study provides a foundational approach for B cells-based tumor classification in HNSCC, offering insights into targeted treatment and immunotherapy strategies.

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来源期刊
International Journal of Oral Science
International Journal of Oral Science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
31.80
自引率
1.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: The International Journal of Oral Science covers various aspects of oral science and interdisciplinary fields, encompassing basic, applied, and clinical research. Topics include, but are not limited to: Oral microbiology Oral and maxillofacial oncology Cariology Oral inflammation and infection Dental stem cells and regenerative medicine Craniofacial surgery Dental material Oral biomechanics Oral, dental, and maxillofacial genetic and developmental diseases Craniofacial bone research Craniofacial-related biomaterials Temporomandibular joint disorder and osteoarthritis The journal publishes peer-reviewed Articles presenting new research results and Review Articles offering concise summaries of specific areas in oral science.
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