GC/MS Analysis and Isolation of Stigmasterol from Viola Odorata Cultivated in Iraq

Viola odorata is a viola family flowering plant native to Europe and Asia. This is a small, hardy, herbaceous, perennial plant. It is also known as sweet violet and English violet. Numerous pharmacological properties of this flowering plant include anticancer, antibacterial, anti-inflammatory, antioxidant and antipyretic properties. Previous literature review of different parts of Viola odorata resulted in detection of different chemical constituents such as flavonoids (Quercetin, kaempferol), glycosides (Rutin) and terpenoids (stigma sterol). Stigmasterol was detected using TLC and GC-MS in this study. It was isolated from Viola odorata using preparative layer chromatography. HPLC and FTIR analysis confirmed the isolated Stigmasterol