HPTLC 植物化学成分分析及槲皮素和没食子酸的同时定量

Q3 Pharmacology, Toxicology and Pharmaceutics

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI:10.52711/0974-360x.2024.00042

Anant Kumar Srivastav, Shikhar Verma, Himani Awasthi, Santosh Kumar

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引用次数: 0

摘要

通过对俗称 "卡当布 "的 Neolamarckia cadamba (Roxb.) Bosser 进行 HPTLC 分析，发现在测试样品的甲醇提取物中存在多种生物活性化合物以及一定比例的槲皮素和没食子酸。通过 HPTLC CAMAG 扫描仪 III，在 254nm 和 366nm 波长下对 Neolamarckia cadamba 的标记物（槲皮素和没食子酸）进行了定量，并进行了植物化学分析。利用峰面积和峰高进行定量。色谱图和轨迹显示，测试样品 1 和样品 2 的槲皮素含量分别为 1.29% 和 0.77%。根据色谱图中出现的峰值数量，样品 1 和样品 2 的植物化学成分分析表明分别含有 16 和 10 种未知生物活性化合物。由于这项研究确认了植物化学成分分析，槲皮素和没食子酸的定量分析将有助于进一步研究霓裳草的药理和治疗价值。

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HPTLC Phytochemical Profiling and Simultaneous Quantification of Quercetin and Gallic acid in Neolamarckia cadamba (Roxb.)

HPTLC analysis of Neolamarckia cadamba (Roxb.) Bosser commonly known as kadamb reveals presence of several bioactive compounds along with percentage of quercetin and gallic acid in the methanolic extract of test samples. Quantification of markers (quercetin and gallic acid) along with phytochemical profiling of Neolamarckia cadamba was done at 254nm and 366nm wavelength through HPTLC CAMAG scanner III. Quantification was done by using peak area and peak height. Chromatograms and tracks obtained reveals that the test samples, sample 1 and sample 2 have quercetin 1.29% and 0.77% respectively. Phytochemical profiling of sample 1 and sample 2 reveals presence of 16 and 10 unknown bioactive compounds respectively denoted by the number of peaks present in chromatograms. As this study acknowledging phytochemical profiling, quantification of quercetin and gallic acid will be accountable for further research towards pharmacognostic and therapeutic value of Neolamarckia cadamba.

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来源期刊

Research Journal of Pharmacy and Technology Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal, devoted to pharmaceutical sciences. The aim of RJPT is to increase the impact of pharmaceutical research both in academia and industry, with strong emphasis on quality and originality. RJPT publishes Original Research Articles, Short Communications, Review Articles in all areas of pharmaceutical sciences from the discovery of a drug up to clinical evaluation. Topics covered are: Pharmaceutics and Pharmacokinetics; Pharmaceutical chemistry including medicinal and analytical chemistry; Pharmacognosy including herbal products standardization and Phytochemistry; Pharmacology: Allied sciences including drug regulatory affairs, Pharmaceutical Marketing, Pharmaceutical Microbiology, Pharmaceutical biochemistry, Pharmaceutical Education and Hospital Pharmacy.