脐带血在纠正应激引起的大鼠高血压变化中的作用

L. Samokhina, V. Lomako, Yu.S. Rudyk
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摘要

脐带血间充质干细胞(CB)被积极用于治疗心血管疾病,其中起重要作用的是能在人体内形成血管紧张素 II 的糜蛋白酶和 tonin(或卡利克雷因 II)。在老年人中,在糜蛋白酶活性降低的背景下,血管紧张素 II 的作用会导致血压和心率升高。我们的工作旨在研究向患有应激性高血压(SIH)的老年大鼠注射异基因 CB 时,糜蛋白酶和 tonin 的活性。SIH 采用 "不回避 "试验进行模拟,每天进行一次,持续三周,直到高血压稳定为止。异体低温保存的 CB 取自 17-19 天大的大鼠胚胎,腹腔注射一次,每次 0.5 毫升(3.5∙107 个细胞/毫升)。注射4天后,用酶法测定血清、脑皮质、肺、心、肝和肾组织无核匀浆中糜蛋白酶和冬眠素的活性。SIH导致糜蛋白酶活性降低,血清、脑皮质和肾脏中的糜蛋白酶活性显著降低,脑皮质、心脏和肾脏中的tonin活性显著降低。向患有 SIH 的大鼠注射 CB 后,除肝脏外,所有样本中的糜蛋白酶和 tonin 活性都增加了。只有大脑皮层和肾脏中的 tonin 发生了显著变化。同时,在脑皮质中未观察到该指标的正常化,这表明需要增加细胞药物的剂量或注射次数,并延长观察时间,以达到全面的更新效果。因此,向 24 个月大的 SIH 大鼠注射同种异体脐带 CB 可使大多数研究组织中的糜蛋白酶和冬眠素活性得到恢复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CORD BLOOD IN CORRECTING STRESS-INDUCED HYPERTENSIVE CHANGES IN RATS
Mesenchymal stem cells from cord blood (CB) are actively used for the correction of cardiovascular disorders, the important role in the formation of which belongs to chymase and tonin (or kallikrein II), capable of forming angiotensin II in humans. In elderly people, the action of tonin leads to an increase in blood pressure and heart rate against the background of chymase activity decrease. The aim of our work was to investigate the activity of chymase and tonin under allogenic CB injection to old rats with stress-induced hypertension (SIH). The SIH was modeled using the “non-avoidance” test, conducting one session daily for three weeks until stable hypertension was achieved. Allogeneic cryopreserved CB, which was obtained from 17-19-day-old rat embryos, was injected intraperitoneally once in 0.5 ml (3.5∙107 cells/ml). 4 days after the injection, the activity of chymase and tonin was determined by enzymatic methods in blood serum, nuclear-free homogenates of brain cortex, lung, heart, liver, and kidney tissues. The SIH development led to a decrease in the chymase activity, more significantly in blood serum, brain cortex, kidneys and the tonin activity in the brain cortex, heart and kidneys. After the CB injection to rats with SIH, the chymase and tonin activities increased in all samples except the liver. Significant changes were noted only for tonin in the brain cortex and kidneys. At the same time, normalization of this indicator was not observed in the brain cortex, which indicates the need to increase the dose of the cellular drug or the number of injections and prolong the observation period to achieve a full renewing effect. Thus, allogeneic umbilical CB injection to 24-month-old rats with SIH leads to restoration of chymase and tonin activity in most of the studied tissues.
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