各种碳源对香蕉变种 Chinichampa (AAB) 通过芽尖进行离体培养和芽增殖的影响

S. K. Ozukum, C. S. Maiti, Khamrang Mathukmi
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引用次数: 0

摘要

香蕉是世界市场上最重要的热带水果之一,是重要的主食和主要出口商品。体外繁殖的香蕉植株无病虫害、均匀一致,而且可以快速繁殖,因此正逐渐成为首选的种植材料。本调查于 2019-2022 年期间在那加兰大学 Medziphema 校区农业科学学院园艺系组织培养实验室进行。对不同参数记录的数据,即返青天数、多芽萌发所需天数、每个外植体产生的多芽数量以及30天后多芽的长度,进行了完全随机设计(CRD)。本研究揭示了各种碳源的重要性及其在通过芽尖培养进行香蕉微繁殖中的作用。在添加了 30 克蔗糖的芽增殖培养基(SPM S)中,观察到了最短的返青天数、多芽萌发和最长的多芽长度。在添加了 30 克食糖的芽增殖培养基中,多芽数量最多。结果表明,蔗糖作为香蕉微繁殖的碳源具有重要意义。它们提高了植株再生能力,促进了无病无病毒香蕉植株的快速繁殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Response of various carbon sources for in vitro culture establishment and shoot proliferation of banana cv. Chinichampa (AAB) through shoot tip 
Bananas are one of the most important tropical fruits in the world market, a significant staple food and a major export commodity. In vitro propagated banana plants are steadily becoming the planting material of choice because of disease free, uniformity and the possibility of rapid multiplication. The present investigation was carried out at the tissue culture laboratory, Department of Horticulture, School of Agricultural Sciences, Nagaland University, Medziphema campus during the year 2019-2022. Data recorded for different parameters viz. days to greening, days required for multiple buds’ initiation, number of multiple buds produced per explant and length of multiple buds after 30 days were subjected to completely randomized design (CRD). The present study revealed the importance of various carbon sources and its role in micropropagation of banana through shoot tip culture. The minimum day to greening, multiple buds initiation and maximum length of multiple buds was observed in shoot proliferation medium supplemented (SPM S) with 30g sucrose. The highest number of multiple buds was recorded in shoot proliferation medium supplemented with 30g table sugar. The results indicate the significance of sucrose as carbon sources in micropropagation of banana. They improved plant regeneration and facilitated rapid multiplication of disease and virus free banana plantlets.
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