通过 MTT 分析法体外评估 Karanthai Legium（Kl）--一种治疗宫颈癌的 Siddha 药物--对 Hela 细胞株的抗癌活性

Q3 Pharmacology, Toxicology and Pharmaceutics

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI:10.52711/0974-360x.2024.00125

Carolin P, M. A, Meena Kumari R

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引用次数: 0

摘要

宫颈癌已成为全球的主要负担，当然也被认为是妇女（尤其是发展中国家的妇女）因癌症死亡的主要原因之一。人类乳头瘤病毒（HPV）的病毒诱导会扰乱细胞控制生长的基本机制。目前，药用植物已成为治疗癌症研究的药物发现来源。释迦医学是印度的一种古老医学体系，它将疾病分为 4448 种类型。在悉达文献中，癌症被称为 Vippuruthinoipadalam（类似癌症的疾病）、Kiranthi noi、Mega Katti、Pilavai 和 Putru noi（肿瘤）。文献中收集了大量治疗癌症的草药、矿物药和矿物药。许多抗癌药物现在都在悉达医学中使用。其中，Karanthai Legium（KL）是释迦文献中提到的一种具有抗癌潜力的经典矿物药。本研究的主要目的是利用 MTT 法评估 Karantha Legium 对 HeLa 细胞的抗癌活性。目前的研究表明，Karanthailegium（KL）的浓度高达 200µg/ml 时，对 HeLa 细胞株的细胞毒性活性高达 72.44%。经计算，KL 对 HeLa 细胞的 IC50 值为 104.90µg/ml。本研究结果表明，制剂 KL 具有良好的抗癌活性。

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In vitro Evaluation of Anticancer Activity of Karanthai Legium (Kl) – A Siddha Medicine for Cervical Carcinoma against Hela Cell Line By MTT Assay

Cervical cancer become a major global burden and of course considered one of the leading causes of cancer-related deaths in women, particularly in developing countries. Viral induction of human papilloma viruses (HPV) can disturb the basic cellular mechanism of growth control. Currently medicinal plants have become the source of drug discovery in research for treating cancer. Siddha medicine is an ancient medical system of India which classifies diseases into 4448 types. In Siddha literature, Cancer is mentioned as Vippuruthinoipadalam (Carcinoma like illness), Kiranthi noi, Mega Katti, Pilavai and Putru noi (tumour). Literary evidences have a classic collection of herbal, mineral, herbo- mineral medicines for Cancer. Many anti-cancerous drugs are now in practice in Siddha medicine. Among them, Karanthai Legium (KL) a classical herbo mineral drug having anticancer potential is mentioned in Siddha Literature. The main objective of this study is to evaluate anti- cancer activity against HeLa cell using MTT assay. Present study revealed that, Karanthailegium (KL) at high as 200µg/ml showed high cytotoxicity activity as high as 72.44% against HeLa cell lines. IC50 of the tested KL against HeLa cells was calculated as104.90µg/ml. It was concluded from the result of the present study that the formulation KL possesses promising anti-cancer activity.

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来源期刊

Research Journal of Pharmacy and Technology Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal, devoted to pharmaceutical sciences. The aim of RJPT is to increase the impact of pharmaceutical research both in academia and industry, with strong emphasis on quality and originality. RJPT publishes Original Research Articles, Short Communications, Review Articles in all areas of pharmaceutical sciences from the discovery of a drug up to clinical evaluation. Topics covered are: Pharmaceutics and Pharmacokinetics; Pharmaceutical chemistry including medicinal and analytical chemistry; Pharmacognosy including herbal products standardization and Phytochemistry; Pharmacology: Allied sciences including drug regulatory affairs, Pharmaceutical Marketing, Pharmaceutical Microbiology, Pharmaceutical biochemistry, Pharmaceutical Education and Hospital Pharmacy.