草药提取物甘草和柑橘对斑马鱼（Danio rerio）的致畸胎作用和美白效果比较

Q3 Pharmacology, Toxicology and Pharmaceutics

Research Journal of Pharmacy and Technology Pub Date : 2024-02-20 DOI:10.52711/0974-360x.2024.00102

Shaikh Saba, Abusufiyan Shaikh, Ajaz Ahmed, Nameerah Rakhe, Trupti Parab, Saniya Khan

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引用次数: 0

摘要

目的确定甘草和正柑的甲醇提取物对斑马鱼的急性毒性和抗黑色素生成活性的协同效应。材料和方法根据 OECD 准则 236 研究了鱼胚胎毒性（FET）。计算了两种提取物的 LC50 和最大非致死浓度。使用 labomed 数码显微镜拍摄暴露于不同浓度甲醇提取物及其组合的斑马鱼幼体图像。使用 imageJ 软件测量图像的综合密度，研究提取物的皮肤美白效果：发现甘草和正柑的甲醇提取物的半致死浓度和最大非致死浓度分别为 56.23ppm 和 34.7ppm。结果表明，暴露于甘草和正南橼甲醇提取物的斑马鱼胚胎孵化率明显降低（甘草p<0.0014，正南橼p<0.0011），死亡率随剂量增加，并出现不同的发育形态异常。研究发现，草药提取物的协同作用在降低黑色素含量方面更为有效。结论极高浓度的甘草和正柑对斑马鱼胚胎有致畸作用。不过，根据 OECD 水生毒性分类，这两种提取物都是安全的。我们的研究还发现，这两种提取物对斑马鱼幼体都有协同增白皮肤的作用，这可能是由于这些提取物降低了褪黑激素的含量。

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Comparative Teratogenic and Skin whitening effect of Herbal extract Glycyrrhiza glabra and Citrus sineus on Zebrafish (Danio rerio)

Objective: To determine acute toxicity and Synergistic effect of the anti-melanogenic activity of methanolic extract Glycyrrhiza glabra and Citrus sineus on zebrafish. Materials and method: Fish embryo toxicity (FET) was studied as per OECD guidelines 236. The LC50 and maximum non-lethal concentration of both the extracts were calculated. The images of zebrafish larvae exposed to different concentrations of methanolic extracts and their combination were taken by using labomed digital microscope. The skin whitening effect of extract was studied by measuring integrated density of images by using imageJ software Result: LC50 Value and maximum non-lethal concentration of methanolic extracts of Glycyrrhiza glabra and Citrus sineus were found to be 56.23ppm and 34.7ppm respectively. The result revealed significant reduction in percentage hatchability (p < 0.0014 for glycyrrhiza glabra and p<0.0011 for Citrus sinenus), dose dependant increased in percentage mortality and different developmental morphological abnormalities in zebrafish embryos exposed to the methanolic extracts Glycyrrhiza glabra and Citrus sineus. The synergistic effect of herbal extracts was found to be more effective in reducing the melanin content. Conclusion: Very High concentration of Glycyrrhiza glabra and Citrus Sineus showed teratogenic effect in zebrafish embryoes. However, based on OECD aquatic toxicity classification, both the extracts were found to be safe. Our study also revealed synergestic skin whitening effect of both the extracts in zebrafish larvae which might be due to reduction of melatonin content by these extracts.

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来源期刊

Research Journal of Pharmacy and Technology Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal, devoted to pharmaceutical sciences. The aim of RJPT is to increase the impact of pharmaceutical research both in academia and industry, with strong emphasis on quality and originality. RJPT publishes Original Research Articles, Short Communications, Review Articles in all areas of pharmaceutical sciences from the discovery of a drug up to clinical evaluation. Topics covered are: Pharmaceutics and Pharmacokinetics; Pharmaceutical chemistry including medicinal and analytical chemistry; Pharmacognosy including herbal products standardization and Phytochemistry; Pharmacology: Allied sciences including drug regulatory affairs, Pharmaceutical Marketing, Pharmaceutical Microbiology, Pharmaceutical biochemistry, Pharmaceutical Education and Hospital Pharmacy.