{"title":"阿特温(土耳其)特有的金钟罩(Campanula choruhensis Kit Tan & Sorger)的 DNA 条形码","authors":"Hayal AKYILDIRIM BEĞEN, Özgür Emi̇nağaoğlu, Melahat Özcan","doi":"10.31195/ejejfs.1401687","DOIUrl":null,"url":null,"abstract":"DNA barcoding is the method of description of species based on gene diversity. In current studies, registration, genetic identification and protection of especially endemic plant species are carried out by DNA barcoding techniques. Molecular studies are based on the amplification and sequencing of the barcode gene region by the PCR method. Campanula choruhensis Kit Tan & Sorger is endemic and widespread in Artvin, Erzurum and around Çoruh valley passing through it. Roots of this species are used in medicinal and aromatic studies. Intense roadworks and dam constructions are carried out in and around the distribution area of this species. This situation harms the habitat of the species and comes across with its extinction. In this study, the plastid barcode gene regions (trnK-rps16 and trnL-rpl32) of the C. choruhensis was sequenced from five populations. To make the identification of this species quickly and accurately, gene sequence compared with sequences of other related Campanula L. species. In addition, gene sequences and phylogenetic relations were given.","PeriodicalId":197799,"journal":{"name":"Eurasian Journal of Forest Science","volume":"35 16","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Artvin (Türkiye) Endemiği Campanula choruhensis Kit Tan & Sorger’in DNA Barkodlaması\",\"authors\":\"Hayal AKYILDIRIM BEĞEN, Özgür Emi̇nağaoğlu, Melahat Özcan\",\"doi\":\"10.31195/ejejfs.1401687\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"DNA barcoding is the method of description of species based on gene diversity. In current studies, registration, genetic identification and protection of especially endemic plant species are carried out by DNA barcoding techniques. Molecular studies are based on the amplification and sequencing of the barcode gene region by the PCR method. Campanula choruhensis Kit Tan & Sorger is endemic and widespread in Artvin, Erzurum and around Çoruh valley passing through it. Roots of this species are used in medicinal and aromatic studies. Intense roadworks and dam constructions are carried out in and around the distribution area of this species. This situation harms the habitat of the species and comes across with its extinction. In this study, the plastid barcode gene regions (trnK-rps16 and trnL-rpl32) of the C. choruhensis was sequenced from five populations. To make the identification of this species quickly and accurately, gene sequence compared with sequences of other related Campanula L. species. In addition, gene sequences and phylogenetic relations were given.\",\"PeriodicalId\":197799,\"journal\":{\"name\":\"Eurasian Journal of Forest Science\",\"volume\":\"35 16\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-02-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Eurasian Journal of Forest Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31195/ejejfs.1401687\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eurasian Journal of Forest Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31195/ejejfs.1401687","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
DNA 条形码是根据基因多样性描述物种的方法。在目前的研究中,特别是地方性植物物种的登记、基因鉴定和保护都是通过 DNA 条形码技术进行的。分子研究是通过 PCR 方法对条形码基因区域进行扩增和测序。金钟罩(Campanula choruhensis Kit Tan & Sorger)是埃尔祖鲁姆阿特温和经过此地的乔鲁赫山谷(çoruh valley)附近的特有种,分布广泛。该物种的根可用于药用和芳香研究。在该物种的分布区及其周围,正在进行密集的道路工程和水坝建设。这种情况破坏了该物种的栖息地,并导致其灭绝。在这项研究中,对五种 Choruhensis 的质体条形码基因区(trnK-rps16 和 trnL-rpl32)进行了测序。为了快速准确地鉴定该物种,将其基因序列与其他相关凌霄花物种的序列进行了比较。此外,还给出了基因序列和系统发育关系。
Artvin (Türkiye) Endemiği Campanula choruhensis Kit Tan & Sorger’in DNA Barkodlaması
DNA barcoding is the method of description of species based on gene diversity. In current studies, registration, genetic identification and protection of especially endemic plant species are carried out by DNA barcoding techniques. Molecular studies are based on the amplification and sequencing of the barcode gene region by the PCR method. Campanula choruhensis Kit Tan & Sorger is endemic and widespread in Artvin, Erzurum and around Çoruh valley passing through it. Roots of this species are used in medicinal and aromatic studies. Intense roadworks and dam constructions are carried out in and around the distribution area of this species. This situation harms the habitat of the species and comes across with its extinction. In this study, the plastid barcode gene regions (trnK-rps16 and trnL-rpl32) of the C. choruhensis was sequenced from five populations. To make the identification of this species quickly and accurately, gene sequence compared with sequences of other related Campanula L. species. In addition, gene sequences and phylogenetic relations were given.