Caroline Mitiká Watanabe, Nathália Almeida Souza Tancler, Aline Márcia Marques Braz, Shelly Favorito de Carvalho, Giovanni Faria Silva, Maria Inês de Moura Campos Pardini, Maria Aparecida Custódio Domingues, Guilherme Targino Valente, Paulo Eduardo de Abreu Machado, Rejane Maria Tommasini Grotto, Marjorie de Assis Golim
{"title":"受丙型肝炎病毒感染的人类巨核细胞和血小板：细胞内和细胞外评估","authors":"Caroline Mitiká Watanabe, Nathália Almeida Souza Tancler, Aline Márcia Marques Braz, Shelly Favorito de Carvalho, Giovanni Faria Silva, Maria Inês de Moura Campos Pardini, Maria Aparecida Custódio Domingues, Guilherme Targino Valente, Paulo Eduardo de Abreu Machado, Rejane Maria Tommasini Grotto, Marjorie de Assis Golim","doi":"10.34257/gjmrcvol24is1pg1","DOIUrl":null,"url":null,"abstract":"This study aimed to determine whether the hepatitis C virus (HCV) infects human megakaryocytes and platelets and to measure the expression of receptors involved in virus-cell interaction. Platelets from healthy donors were infected with HCV in vitro and analyzed for viral expression and the receptors claudin-1 and cluster of differentiation 81 (CD81). HCV was detected on the surface and cytoplasm of both cells; cytoplasmic expression was higher compared to the surface. Platelets presented a claudin-1+/CD81-phenotype, and megakaryocytes showed aclaudin-1+/CD81+ phenotype. We conclude that megakaryocytes and platelets are susceptible to HCV infection, regardless of CD81 expression, and megakaryocytes may serve as possible sites of viral replication.","PeriodicalId":93101,"journal":{"name":"Global journal of medical research","volume":"34 5","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hepatitis C Virus Infected Human Megakaryocytes and Platelets: Intra-and Extracellular Evaluation\",\"authors\":\"Caroline Mitiká Watanabe, Nathália Almeida Souza Tancler, Aline Márcia Marques Braz, Shelly Favorito de Carvalho, Giovanni Faria Silva, Maria Inês de Moura Campos Pardini, Maria Aparecida Custódio Domingues, Guilherme Targino Valente, Paulo Eduardo de Abreu Machado, Rejane Maria Tommasini Grotto, Marjorie de Assis Golim\",\"doi\":\"10.34257/gjmrcvol24is1pg1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aimed to determine whether the hepatitis C virus (HCV) infects human megakaryocytes and platelets and to measure the expression of receptors involved in virus-cell interaction. Platelets from healthy donors were infected with HCV in vitro and analyzed for viral expression and the receptors claudin-1 and cluster of differentiation 81 (CD81). HCV was detected on the surface and cytoplasm of both cells; cytoplasmic expression was higher compared to the surface. Platelets presented a claudin-1+/CD81-phenotype, and megakaryocytes showed aclaudin-1+/CD81+ phenotype. We conclude that megakaryocytes and platelets are susceptible to HCV infection, regardless of CD81 expression, and megakaryocytes may serve as possible sites of viral replication.\",\"PeriodicalId\":93101,\"journal\":{\"name\":\"Global journal of medical research\",\"volume\":\"34 5\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-02-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Global journal of medical research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.34257/gjmrcvol24is1pg1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Global journal of medical research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34257/gjmrcvol24is1pg1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Hepatitis C Virus Infected Human Megakaryocytes and Platelets: Intra-and Extracellular Evaluation
This study aimed to determine whether the hepatitis C virus (HCV) infects human megakaryocytes and platelets and to measure the expression of receptors involved in virus-cell interaction. Platelets from healthy donors were infected with HCV in vitro and analyzed for viral expression and the receptors claudin-1 and cluster of differentiation 81 (CD81). HCV was detected on the surface and cytoplasm of both cells; cytoplasmic expression was higher compared to the surface. Platelets presented a claudin-1+/CD81-phenotype, and megakaryocytes showed aclaudin-1+/CD81+ phenotype. We conclude that megakaryocytes and platelets are susceptible to HCV infection, regardless of CD81 expression, and megakaryocytes may serve as possible sites of viral replication.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
