使用快速、准确、经济高效的 QiaXcel 高级平台检测伊拉克北部结直肠癌中的微卫星不稳定性

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL

Journal of Contemporary Medical Sciences Pub Date : 2024-02-26 DOI:10.22317/jcms.v10i1.1469

Nigar A Abdalrahman, Dlnya A. Mohamad

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引用次数: 0

摘要

研究目的这项研究的目的是解决最流行的微卫星不稳定性（MSI）检测方法（使用荧光毛细管测序仪）的局限性：方法：使用基于毛细管电泳技术的QiaXcel Advanced系统（Qiagen，Hilden，Germany）检测了53份伊拉克正火固定石蜡包埋（FFPE）CRC样本的MSI状态。使用的十二个多态标记包括 BAT25、BAT26、BAT40、D2S123、D5S346、D17S250、NR21、NR22、NR27、Mycl1、TPOX 和 TH01：使用 QiaXcel Advanced 平台成功确定了 MSI 状态。在 53 例 CRC 中，有 12 例（22.64%）发现了 MSI，其中有 MSI-H：结论：由于 MSI 在癌症进展中的重要作用，这种基于 PCR 的快速、廉价技术可以加强对 CRC 的临床管理，从而进一步改变患者的预后。

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Using a Rapid, Accurate, and Cost-Effective QiaXcel Advanced Platform for Microsatellite Instability Detection in Colorectal Carcinoma in North of Iraq

Objective: The objective of this investigation was to address the limitations of the most popular microsatellite instability (MSI) detectionmethod, which uses fluorescent capillary sequencers. Methods: Using the QiaXcel Advanced system (Qiagen, Hilden, Germany) based on capillary electrophoresis, the MSI status of 53 IraqiFormalin Fixed Paraffin Embedded (FFPE) CRC samples was examined. BAT25, BAT26, BAT40, D2S123, D5S346, D17S250, NR21, NR22, NR27,Mycl1, TPOX, and TH01 were among the panel of twelve polymorphic markers that were used. Results: Using a QiaXcel Advanced platform was successfully established to determine the MSI status. Among 53 cases of CRC, MSI wasobserved in 12 cases (22.64%) who had MSI-H. Conclusion: Due to MSI’s significance in the progression of cancer, this quick and inexpensive PCR-based technique can enhance theclinical management of CRC, which may further alter the patient’s outcome.

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Journal of Contemporary Medical Sciences MEDICINE, GENERAL & INTERNAL-

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0.00%

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审稿时长

12 weeks