利用农用工业废料从苏云金芽孢杆菌 KUMBNGBT-41 中提取聚羟基丁酸盐 (PHB) 的生物勘探

IF 0.2 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
G. Nandish, S. Akarsh, D. Manjunatha, H. V. Sowmya, B. Thippeswamy
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引用次数: 0

摘要

众所周知,用于制造塑料的各种化学物质具有剧毒,对生物圈构成严重威胁。这些物质除了对生态系统造成严重破坏外,还会引发一系列问题,如先天缺陷、癌症、神经和免疫系统损伤等。本研究工作旨在从 Chitradurga 地区 Sanehally 收集的垃圾填埋场土壤样本中分离并鉴定产生 PHB 的细菌,并使用活体染色技术进行定性筛选。采用溶剂萃取法进行定量评估。根据形态学、生物化学和分子鉴定对产生 PHB 的细菌进行了表征,确认其为苏云金芽孢杆菌菌株 KUMBNGBT-41(登录号:MW040075)。在培养基-营养肉汤、培养时间-72 小时、温度-37˚C、pH-7.0、碳源-葡萄糖、氮源-氯化铵和碳氮比为 4:1 等不同条件下,对生物量生产的生长参数进行了优化。低成本生产培养基用于在浸没式发酵中使用较便宜的底物生产 PHB。使用紫外可见分光光度计在 200-600nm 波长范围内对 PHB 进行分析和定量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioprospecting of Polyhydroxy butyrate (PHB) from Bacillus thuringiensis KUMBNGBT-41 by using Agro-industrial wastes
The diverse group of chemicals used in making of plastic is known to be highly toxic and poses a serious threat to the biosphere. These substances besides hitting hard to ecosystem cause an array of problems like birth defects, cancer, damage of nervous and immune systems. The present research work was aimed to isolate and characterize PHB producing bacteria from landfill soil sample collected from Sanehally, Chitradurga district and qualitatively screened by using viable staining techniques. Quantitative estimation was examined by using solvent extraction method. The PHB producing bacteria were characterized based on morphological, biochemical and molecular identification and confirmed as Bacillus thuringiensis strain KUMBNGBT-41 (Accession No. MW040075). Growth parameters were optimized for biomass production using various conditions such as media-nutrient broth, incubation time-72hrs, temperature-37˚C, pH-7.0, carbon source-glucose, nitrogen source-ammonium chloride and carbon to nitrogen ratio as 4:1. The low-cost production media was used for PHB production by cheaper substrates in submerged fermentation. PHB was analysed and quantified by using UV-Visible spectrophotometer at 200-600nm.
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来源期刊
Research Journal of Biotechnology
Research Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
0.60
自引率
0.00%
发文量
192
期刊介绍: We invite you to contribute Research Papers / Short Communications / Review Papers: -In any field of Biotechnology, Biochemistry, Microbiology and Industrial Microbiology, Soil Technology, Agriculture Biotechnology. -in any field related to Food Biotechnology, Nutrition Biotechnology, Genetic Engineering and Commercial Biotechnology. -in any field of Biotechnology related to Drugs and Pharmaceutical products for human beings, animals and plants. -in any field related to Environmental Biotechnolgy, Waste Treatment of Liquids, Soilds and Gases; Sustainability. -in inter-realted field of Chemical Sciences, Biological Sciences, Environmental Sciences and Life Sciences. -in any field related to Biotechnological Engineering, Industrial Biotechnology and Instrumentation. -in any field related to Nano-technology. -in any field related to Plant Biotechnology.
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