基于水杨醛和氨基酸衍生的还原席夫碱的铜(II)配合物的 DNA 切割和细胞毒活性

European Pharmaceutical Journal Pub Date : 2024-03-28 DOI:10.2478/afpuc-2024-0003

Jana Korcová, Mária Húserková, Lucia Lintnerová, J. Valentová

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引用次数: 0

摘要

金属复合物在生理条件下具有氧化还原特性，能够与 DNA 结合，是切割 DNA 链的重要工具。这对于将它们用作抗肿瘤药物非常重要。我们合成了来自短链氨基酸和水杨醛的配体。所制备的还原型希夫碱配体随后被用于制备铜（II）配合物。研究的目的是对铜（II）络合物进行体外测试，结果证实它们能够裂解 DNA。在保留健康线粒体功能的基础上，还用雷沙祖林氧化还原法确认了铜(II)络合物对酿酒酵母的细胞毒性活性。

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DNA Cleavage and Cytotoxic Activity of Copper(II) Complexes Based on Reduced Schiff Bases Derived From Salicylaldehyde and Amino Acids

Metal complexes, which, under physiologic conditions, show redox properties and are able to bind to DNA, are great tools to cleave the DNA chain. This aspect is of great importance for their use as antineoplastic drugs. We synthesized ligands derived from short-chain amino acids and from salicylaldehyde. The prepared ligands of the type of reduced Schiff bases were subsequently used for the preparation of copper(II) complexes. The aim of the study was in vitro testing of copper(II) complexes, where it was confirmed that they are capable of cleaving DNA. Their cytotoxic activity was also confirmed by the resazurin redox method on Saccharomyces cerevisiae based on preserved healthy mitochondrial function.

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.