J. Michael Conlon , Ananyaa Sridhar , Dawood Khan , Taylor S. Cunning , Jack J. Delaney , Megan G. Taggart , Nigel G. Ternan , Jérôme Leprince , Laurent Coquet , Thierry Jouenne , Samir Attoub , Milena Mechkarska
{"title":"从香蕉树栖蛙 Boana platanera (Hylidae; Hylinae) 皮肤分泌物中分离出的多功能宿主防御肽。","authors":"J. Michael Conlon , Ananyaa Sridhar , Dawood Khan , Taylor S. Cunning , Jack J. Delaney , Megan G. Taggart , Nigel G. Ternan , Jérôme Leprince , Laurent Coquet , Thierry Jouenne , Samir Attoub , Milena Mechkarska","doi":"10.1016/j.biochi.2024.03.012","DOIUrl":null,"url":null,"abstract":"<div><p>Five host-defense peptides (figainin 2PL, hylin PL, raniseptin PL, plasticin PL, and peptide YL) were isolated from norepinephrine-stimulated skin secretions of the banana tree dwelling frog <em>Boana platanera</em> (Hylidae; Hylinae) collected in Trinidad.</p><p>Raniseptin PL (GVFDTVKKIGKAVGKFALGVAKNYLNS.NH<sub>2</sub>) and figainin 2PL (FLGTVLKLGKAIAKTVVPMLTNAMQPKQ. NH<sub>2</sub>) showed potent and rapid bactericidal activity against a range of clinically relevant Gram-positive and Gram-negative ESKAPE <sup>+</sup> pathogens and <em>Clostridioides difficile.</em> The peptides also showed potent cytotoxic activity (LC<sub>50</sub> values < 30 μM) against A549, MDA-MB-231 and HT29 human tumor-derived cell lines but appreciably lower hemolytic activity against mouse erythrocytes (LC<sub>50</sub> = 262 ± 14 μM for raniseptin PL and 157 ± 16 μM for figainin 2PL). Hylin PL (FLGLIPALAGAIGNLIK.NH<sub>2</sub>) showed relatively weak activity against microorganisms but was more hemolytic. The glycine-leucine-rich peptide with structural similarity to the plasticins (GLLSTVGGLVGGLLNNLGL.NH<sub>2</sub>) and the non-cytotoxic peptide YL (YVPGVIESLL.NH<sub>2</sub>) lacked antimicrobial and cytotoxic activities. Hylin PL, raniseptinPL and peptide YL stimulated the rate of release of insulin from BRIN-BD11 clonal β-cells at concentrations ≥100 nM. Peptide YL was the most effective (2.3-fold increase compared with basal rate at 1 μM concentration) and may represent a template for the design of a new class of incretin-based anti-diabetic drugs.</p></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0300908424000701/pdfft?md5=0e62b58f0bacba03f8bb7c5ea584abbd&pid=1-s2.0-S0300908424000701-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Multifunctional host-defense peptides isolated from skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae)\",\"authors\":\"J. Michael Conlon , Ananyaa Sridhar , Dawood Khan , Taylor S. Cunning , Jack J. Delaney , Megan G. Taggart , Nigel G. Ternan , Jérôme Leprince , Laurent Coquet , Thierry Jouenne , Samir Attoub , Milena Mechkarska\",\"doi\":\"10.1016/j.biochi.2024.03.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Five host-defense peptides (figainin 2PL, hylin PL, raniseptin PL, plasticin PL, and peptide YL) were isolated from norepinephrine-stimulated skin secretions of the banana tree dwelling frog <em>Boana platanera</em> (Hylidae; Hylinae) collected in Trinidad.</p><p>Raniseptin PL (GVFDTVKKIGKAVGKFALGVAKNYLNS.NH<sub>2</sub>) and figainin 2PL (FLGTVLKLGKAIAKTVVPMLTNAMQPKQ. NH<sub>2</sub>) showed potent and rapid bactericidal activity against a range of clinically relevant Gram-positive and Gram-negative ESKAPE <sup>+</sup> pathogens and <em>Clostridioides difficile.</em> The peptides also showed potent cytotoxic activity (LC<sub>50</sub> values < 30 μM) against A549, MDA-MB-231 and HT29 human tumor-derived cell lines but appreciably lower hemolytic activity against mouse erythrocytes (LC<sub>50</sub> = 262 ± 14 μM for raniseptin PL and 157 ± 16 μM for figainin 2PL). Hylin PL (FLGLIPALAGAIGNLIK.NH<sub>2</sub>) showed relatively weak activity against microorganisms but was more hemolytic. The glycine-leucine-rich peptide with structural similarity to the plasticins (GLLSTVGGLVGGLLNNLGL.NH<sub>2</sub>) and the non-cytotoxic peptide YL (YVPGVIESLL.NH<sub>2</sub>) lacked antimicrobial and cytotoxic activities. Hylin PL, raniseptinPL and peptide YL stimulated the rate of release of insulin from BRIN-BD11 clonal β-cells at concentrations ≥100 nM. 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Multifunctional host-defense peptides isolated from skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae)
Five host-defense peptides (figainin 2PL, hylin PL, raniseptin PL, plasticin PL, and peptide YL) were isolated from norepinephrine-stimulated skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae) collected in Trinidad.
Raniseptin PL (GVFDTVKKIGKAVGKFALGVAKNYLNS.NH2) and figainin 2PL (FLGTVLKLGKAIAKTVVPMLTNAMQPKQ. NH2) showed potent and rapid bactericidal activity against a range of clinically relevant Gram-positive and Gram-negative ESKAPE + pathogens and Clostridioides difficile. The peptides also showed potent cytotoxic activity (LC50 values < 30 μM) against A549, MDA-MB-231 and HT29 human tumor-derived cell lines but appreciably lower hemolytic activity against mouse erythrocytes (LC50 = 262 ± 14 μM for raniseptin PL and 157 ± 16 μM for figainin 2PL). Hylin PL (FLGLIPALAGAIGNLIK.NH2) showed relatively weak activity against microorganisms but was more hemolytic. The glycine-leucine-rich peptide with structural similarity to the plasticins (GLLSTVGGLVGGLLNNLGL.NH2) and the non-cytotoxic peptide YL (YVPGVIESLL.NH2) lacked antimicrobial and cytotoxic activities. Hylin PL, raniseptinPL and peptide YL stimulated the rate of release of insulin from BRIN-BD11 clonal β-cells at concentrations ≥100 nM. Peptide YL was the most effective (2.3-fold increase compared with basal rate at 1 μM concentration) and may represent a template for the design of a new class of incretin-based anti-diabetic drugs.
期刊介绍：
Biochimie publishes original research articles, short communications, review articles, graphical reviews, mini-reviews, and hypotheses in the broad areas of biology, including biochemistry, enzymology, molecular and cell biology, metabolic regulation, genetics, immunology, microbiology, structural biology, genomics, proteomics, and molecular mechanisms of disease. Biochimie publishes exclusively in English.
Articles are subject to peer review, and must satisfy the requirements of originality, high scientific integrity and general interest to a broad range of readers. Submissions that are judged to be of sound scientific and technical quality but do not fully satisfy the requirements for publication in Biochimie may benefit from a transfer service to a more suitable journal within the same subject area.
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