Sarmad Muayad Rasheed Al-Bakri, Antonio Magan-Fernandez, Pablo Galindo-Moreno, Francisco O'Valle, Natividad Martin-Morales, Miguel Padial-Molina, Francisco Mesa
{"title":"检测和比较种植体周围炎、牙周炎和健康患者组织样本中的中性粒细胞胞外捕获物：试点研究。","authors":"Sarmad Muayad Rasheed Al-Bakri, Antonio Magan-Fernandez, Pablo Galindo-Moreno, Francisco O'Valle, Natividad Martin-Morales, Miguel Padial-Molina, Francisco Mesa","doi":"10.1111/cid.13325","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>The aim of this study was to detect and compare the tissular expression of neutrophil extracellular traps (NETs) in peri-implant and periodontal samples of patients with peri-implantitis, periodontitis, and controls.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>An observational study was performed on patients with peri-implantitis, periodontitis, and controls. Peri-implant and/or periodontal clinical examinations were performed on each participant. Tissue samples were collected during tooth/implant extraction for clinical reasons. Electron microscopy analysis, Picro-Sirius red staining, immunohistochemical (CD15), and immunofluorescence (citrullinated H3 and myeloperoxidase) techniques were performed to detect NET-related structures and the degree of connective tissue destruction, between the study groups.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Sixty-four patients were included in the study: 28 peri-implantitis, 26 periodontitis, and 10 controls, with a total of 51 implants, 26 periodontal teeth, and 10 control teeth. Neutrophil release of nuclear content was observed in transmission electron microscopy. Immunohistochemical analysis showed a greater CD15 expression in both peri-implantitis and periodontitis compared to controls (<i>p</i> < 0.001), and peri-implantitis presented lower levels of connective tissue and collagen compared to both periodontitis (<i>p</i> = 0.044; <i>p</i> < 0.001) and controls (<i>p</i> < 0.001). Immunofluorescence showed greater citH3 expression in peri-implantitis than the one found in both periodontitis (<i>p</i> = 0.003) and controls (<i>p</i> = 0.048).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>A greater presence and involvement of neutrophils, as well as a greater connective tissue destruction were observed in cases of peri-implantitis. A higher expression of NET-related markers was found in mucosal samples of peri-implantitis compared to periodontitis and controls.</p>\n </section>\n </div>","PeriodicalId":50679,"journal":{"name":"Clinical Implant Dentistry and Related Research","volume":"26 3","pages":"631-641"},"PeriodicalIF":3.7000,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cid.13325","citationCount":"0","resultStr":"{\"title\":\"Detection and comparison of neutrophil extracellular traps in tissue samples of peri-implantitis, periodontitis, and healthy patients: A pilot study\",\"authors\":\"Sarmad Muayad Rasheed Al-Bakri, Antonio Magan-Fernandez, Pablo Galindo-Moreno, Francisco O'Valle, Natividad Martin-Morales, Miguel Padial-Molina, Francisco Mesa\",\"doi\":\"10.1111/cid.13325\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>The aim of this study was to detect and compare the tissular expression of neutrophil extracellular traps (NETs) in peri-implant and periodontal samples of patients with peri-implantitis, periodontitis, and controls.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>An observational study was performed on patients with peri-implantitis, periodontitis, and controls. Peri-implant and/or periodontal clinical examinations were performed on each participant. Tissue samples were collected during tooth/implant extraction for clinical reasons. Electron microscopy analysis, Picro-Sirius red staining, immunohistochemical (CD15), and immunofluorescence (citrullinated H3 and myeloperoxidase) techniques were performed to detect NET-related structures and the degree of connective tissue destruction, between the study groups.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Sixty-four patients were included in the study: 28 peri-implantitis, 26 periodontitis, and 10 controls, with a total of 51 implants, 26 periodontal teeth, and 10 control teeth. Neutrophil release of nuclear content was observed in transmission electron microscopy. Immunohistochemical analysis showed a greater CD15 expression in both peri-implantitis and periodontitis compared to controls (<i>p</i> < 0.001), and peri-implantitis presented lower levels of connective tissue and collagen compared to both periodontitis (<i>p</i> = 0.044; <i>p</i> < 0.001) and controls (<i>p</i> < 0.001). Immunofluorescence showed greater citH3 expression in peri-implantitis than the one found in both periodontitis (<i>p</i> = 0.003) and controls (<i>p</i> = 0.048).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>A greater presence and involvement of neutrophils, as well as a greater connective tissue destruction were observed in cases of peri-implantitis. 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Detection and comparison of neutrophil extracellular traps in tissue samples of peri-implantitis, periodontitis, and healthy patients: A pilot study
Objective
The aim of this study was to detect and compare the tissular expression of neutrophil extracellular traps (NETs) in peri-implant and periodontal samples of patients with peri-implantitis, periodontitis, and controls.
Materials and Methods
An observational study was performed on patients with peri-implantitis, periodontitis, and controls. Peri-implant and/or periodontal clinical examinations were performed on each participant. Tissue samples were collected during tooth/implant extraction for clinical reasons. Electron microscopy analysis, Picro-Sirius red staining, immunohistochemical (CD15), and immunofluorescence (citrullinated H3 and myeloperoxidase) techniques were performed to detect NET-related structures and the degree of connective tissue destruction, between the study groups.
Results
Sixty-four patients were included in the study: 28 peri-implantitis, 26 periodontitis, and 10 controls, with a total of 51 implants, 26 periodontal teeth, and 10 control teeth. Neutrophil release of nuclear content was observed in transmission electron microscopy. Immunohistochemical analysis showed a greater CD15 expression in both peri-implantitis and periodontitis compared to controls (p < 0.001), and peri-implantitis presented lower levels of connective tissue and collagen compared to both periodontitis (p = 0.044; p < 0.001) and controls (p < 0.001). Immunofluorescence showed greater citH3 expression in peri-implantitis than the one found in both periodontitis (p = 0.003) and controls (p = 0.048).
Conclusions
A greater presence and involvement of neutrophils, as well as a greater connective tissue destruction were observed in cases of peri-implantitis. A higher expression of NET-related markers was found in mucosal samples of peri-implantitis compared to periodontitis and controls.
期刊介绍：
The goal of Clinical Implant Dentistry and Related Research is to advance the scientific and technical aspects relating to dental implants and related scientific subjects. Dissemination of new and evolving information related to dental implants and the related science is the primary goal of our journal.
The range of topics covered by the journals will include but be not limited to:
New scientific developments relating to bone
Implant surfaces and their relationship to the surrounding tissues
Computer aided implant designs
Computer aided prosthetic designs
Immediate implant loading
Immediate implant placement
Materials relating to bone induction and conduction
New surgical methods relating to implant placement
New materials and methods relating to implant restorations
Methods for determining implant stability
A primary focus of the journal is publication of evidenced based articles evaluating to new dental implants, techniques and multicenter studies evaluating these treatments. In addition basic science research relating to wound healing and osseointegration will be an important focus for the journal.
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