白斑病、口腔黏膜下纤维化以及白斑病和口腔黏膜下纤维化合并病变中循环外泌体 microRNA 的表达谱。

IF 3.2 Q2 PATHOLOGY
Dinesh Yasothkumar, Pratibha Ramani, Selvaraj Jayaraman, Karthikeyan Ramalingam, W M Tilakaratne
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引用次数: 0

摘要

研究目的本研究旨在阐明循环外泌体miRNA miRNA 21、miRNA 184和miRNA 145在研究群体中的表达情况,研究群体包括(i)白斑病患者;(ii)口腔黏膜下纤维化患者;(iii)口腔黏膜下纤维化伴白斑病患者;(iv)口腔鳞状细胞癌患者;以及(v)健康人:研究设计:对 54 名到萨韦埃塔牙科学院和医院门诊部就诊的患者进行了观察研究。患者被分为三组:第一组:健康人(18 人);第二组:病例组(白斑病、OSMF、白斑病和 OSMF)(18 人);第三组:OSCC(18 人)。实时聚合酶链反应分析评估了 miRNA 21、miRNA 184 和 miRNA 145 的表达谱。统计分析采用 SPSS 软件 23 版进行计算:在单因素方差分析检验中,病例组(白斑病、OSMF、白斑病和 OSMF)、健康组和 OSCC 组之间的三种 miRNA 均有显著的统计学差异(P 结论:OSCC 患者血浆循环外泌体 miRNA 的表达量与病例组之间的差异无统计学意义(P>0.05):血浆循环外泌体 miRNAs miRNA 21、miRNA 145 和 miRNA 184 的表达可作为一种新型的血浆生物标记物,用于对恶性转化风险较高的病例组(白斑病、OSMF、白斑病和 OSMF)患者进行分类。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression Profile of Circulating Exosomal microRNAs in Leukoplakia, Oral Submucous Fibrosis, and Combined Lesions of Leukoplakia and Oral Submucous Fibrosis.

Objectives: This study aims to elucidate the expression of circulating exosomal miRNAs miRNA 21, miRNA 184, and miRNA 145 in the studied groups, including patients with (i) leukoplakia; (ii) oral submucous fibrosis; (iii) oral submucous fibrosis with leukoplakia; (iv) oral squamous cell carcinoma; and (v) healthy individuals.

Study design: An observational study was conducted among 54 patients who reported to the outpatient department of Saveetha Dental College and Hospitals. The patients were divided into three groups: Group I healthy individuals (n = 18), Group II: case group (leukoplakia, OSMF, and leukoplakia and OSMF) (n = 18), and Group III: OSCC (n = 18). Real-time polymerase chain reaction analysis was carried out to assess the expression profiles of miRNA 21, miRNA 184, and miRNA 145. The statistical analysis was calculated using SPSS software version 23.

Results: All three miRNAs showed a statistically significant difference in the one-way ANOVA test between the case group (leukoplakia, OSMF, and leukoplakia and OSMF), healthy group, and OSCC group (p < 0.005). The case group (leukoplakia, OSMF, leukoplakia and OSMF) showed upregulated expression of miRNA 21 and miRNA 184 with threefold change and fourfold change and downregulated expression of miRNA 145 with 1.5-fold change when compared to apparently healthy individuals.

Conclusion: Plasma circulating exosomal miRNAs miRNA 21, miRNA 145, and miRNA 184 expression could be a novel panel of plasma biomarkers to categorise case group (leukoplakia, OSMF, leukoplakia and OSMF) patients with a high risk of malignant transformation.

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来源期刊
CiteScore
5.70
自引率
9.50%
发文量
99
期刊介绍: Head & Neck Pathology presents scholarly papers, reviews and symposia that cover the spectrum of human surgical pathology within the anatomic zones of the oral cavity, sinonasal tract, larynx, hypopharynx, salivary gland, ear and temporal bone, and neck. The journal publishes rapid developments in new diagnostic criteria, intraoperative consultation, immunohistochemical studies, molecular techniques, genetic analyses, diagnostic aids, experimental pathology, cytology, radiographic imaging, and application of uniform terminology to allow practitioners to continue to maintain and expand their knowledge in the subspecialty of head and neck pathology. Coverage of practical application to daily clinical practice is supported with proceedings and symposia from international societies and academies devoted to this field. Single-blind peer review The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.
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