大肠杆菌心磷脂合成酶 C 不同寻常的底物特异性并不需要转录参与的 ymdB 基因的产物。

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-03-26 DOI:10.1016/j.bbalip.2024.159483

Katsuhiro Sawasato, Mikhail Bogdanov

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引用次数: 0

摘要

- 即使在 YmdB 没有转录配对的情况下，ClsC 也能驱动心磷脂（CL）的合成。ymdB-clsC的多聚核苷酸转录和翻译被认为是ClsC发挥全部活性所必需的，但这并不是CL合成或ClsC底物特异性所必需的，ClsC可缩合磷脂酰乙醇胺（PE）和磷脂酰甘油（PG）。clsC 基因的真正起始密码子位于上游 ymdB 基因的开放阅读框内。- 从这个新发现的真实起始密码子开始翻译对 ClsC 在体内和体外发挥其心磷脂合成酶活性至关重要。

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The unusual substrate specificity of Escherichia coli cardiolipin synthase C does not require the product of the transcriptionally engaged ymdB gene

Polycistronic transcription and translation of ymdB-clsC have been thought to be required for full activity of ClsC.

The authentic initiation codon of the clsC gene is present within the open reading frame of the upstream located ymdB gene.

ClsC translated from authentic initiation codon drives cardiolipin (CL) synthesis without transcriptionally paired YmdB.

YmdB is not necessary for the substrate specificity of ClsC utilizing phosphatidylethanolamine (PE) as a co-substrate.

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来源期刊

Biochimica et biophysica acta. Molecular and cell biology of lipids 生物-生化与分子生物学

CiteScore

11.00

自引率

2.10%

发文量

109

审稿时长

53 days

期刊介绍： BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.