氯噻酮对成年斑马鱼生化指标的影响

shreya Patil, Roshan C. D’Souza
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引用次数: 0

摘要

杀虫剂的普遍使用导致非目标生物因意外接触而受到有害影响。新烟碱类杀虫剂因其对节肢动物烟碱受体的高亲和力而在全球广为使用,可有效杀死害虫。与传统杀虫剂相比,新烟碱类杀虫剂对脊椎动物烟碱受体的亲和力较低,因此更安全。最近的研究表明,接触新烟碱类杀虫剂会对包括人类在内的脊椎动物产生毒性。斑马鱼是研究生态毒性的常用模式生物之一。这是首次以成年斑马鱼为研究对象,报告新型新烟碱--氯噻酮对死亡率、肝脏抗氧化应激谱、肝功能谱和脑乙酰胆碱酯酶(AChE)的影响。在两个处理期(96 小时和 21 天)内,对暴露于不同浓度氯噻酮(30 毫克/升、50 毫克/升、70 毫克/升、90 毫克/升、110 毫克/升)的五个组和对照组进行了观察。虽然没有观察到死亡现象,但接触氯噻酮会导致超氧化物歧化酶(SOD)活性增加、脂质过氧化、过氧化氢酶(CAT)和谷胱甘肽转移酶(GST)活性降低。处理组的肝酶丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)和酸性磷酸酶(ACP)的浓度也有所增加。这些结果表明,氯噻酮会扰乱肝功能。在脑 AChE 中观察到的显著下降趋势表明,Clothianidin 对成年斑马鱼的 AChE 有抑制作用。为了解 Clothianidin 的确切作用机制,可进一步开展 DNA 损伤和基因表达研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Clothianidin on Biochemical Parameters of Adult Zebrafish
The ubiquitous use of insecticides leads to detrimental effects on non-target organisms due to accidental exposure. Neonicotinoid insecticides are popularly used worldwide for their high affinity for arthropod nicotinic receptors which effectively kill insect pests. Low affinity towards vertebrate nicotinic receptors, make them safer as compared to traditional insecticides. Recent studies demonstrated that neonicotinoid exposure can cause some toxicity in vertebrates including humans. Zebrafish is one of the popular model organisms to study ecotoxicity. This is the first study on adult zebrafish to report the effect of novel neonicotinoid, Clothianidin on mortality, liver antioxidant stress profile, liver function profile and brain acetylcholinesterase (AChE). Observations were made over two treatment periods, 96 hours, and 21 days, in five groups exposed to varying concentrations of Clothianidin viz. 30mg/L, 50mg/L, 70mg/L, 90mg/L, 110mg/L and a control. Although no mortality was observed, Clothianidin exposure led to increased activity of superoxide dismutase (SOD) enzyme, lipid peroxidation and decreased catalase (CAT), glutathione-S-transferase (GST). Treated groups also showed increased concentrations of liver enzyme alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and acid phosphatase (ACP). These results indicate that Clothianidin causes disturbances in the hepatic function. A prominent decreasing trend observed in brain AChE shows that Clothianidin inhibits AChE in adult zebrafish. Further investigations on DNA damage and gene expression studies could be conducted to understand the exact mechanism of action of Clothianidin
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