印度喀拉拉邦中部一家三级医疗中心从儿童中分离出的耐头孢菌素大肠埃希菌和肺炎克雷伯菌的分子特征

Q4 Medicine

Anti-Infective Agents Pub Date : 2024-03-14 DOI:10.2174/0122113525296665240304071400

Santhosh J. Thattil, Suresh Dhanaraj, T. Ajith

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引用次数: 0

摘要

该研究旨在确定从十岁以下儿童中分离出的产生耐头孢菌素的大肠埃希菌和肺炎克雷伯菌中的广谱β-内酰胺酶（ESBL）的分子特征。一项横断面研究分析了对头孢菌素耐药的大肠埃希菌和肺炎克雷伯菌株中的 ESBL 基因，这些菌株是从一家三级医疗中心微生物实验室 2021 年 5 月至 2022 年 7 月期间收到的样本中分离出来的。这些菌株显示出对 ESBL 和头孢菌素的耐药性，并对 bla（β-内酰胺酶）CTX-M-1、blaCTX-M-15、blaCTX-M-U、blaTEM、blaPER 和 SHV 等基因进行了基于 PCR 的基因分型。在分析的 228 个样本中，有 136 个样本（占 60%）没有生长，92 个样本（占 40%）出现了大肠杆菌和肺炎双球菌的生长。在出现生长的分离物中，39 个（42%）对第三代头孢菌素敏感，其余 53 个（57%）对第三代头孢菌素耐药。在出现耐药性的分离株中，22 株（42%）ESBL 阳性，31 株（58%）ESBL 阴性。在 ESBL 阳性的菌株中，9 株大肠杆菌（60%）对 CTX-M-15 和 CTX-M-1 呈阳性。从 10 岁以下儿童标本中分离出的大肠杆菌和肺炎双球菌中，有 41-42% 产生 ESBL。大肠杆菌中常见的产 ESBL 基因是 CTX-M-15 和 CTX-M-1。在产ESBL的肺炎双球菌中，CTX-M-15和CTX-M-U也很普遍。

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Molecular Characteristics of Cephalosporin Resistant Escherichia coli and Klebsiella pneumoniae Isolated from Children in a Tertiary Care Centre of Central Kerala, India

The study was aimed to determine the molecular characteristics of extended-spectrum beta-lactamases (ESBL) producing cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolated from children below ten years of age. Geographically diverse variations in the prevalence of ESBL genes were reported. No data were available on the prevalence of ESBL genes in central Kerala, India, among children below 10 years of age. A cross-sectional study was performed to analyze ESBL genes in cephalosporin-re-sistant E. coli and K. pneumoniae strains isolated from samples received in the Microbiology la-boratory of a tertiary care centre during the period between May 2021 and July 2022. The strains showed that ESBL + cephalosporin resistance was subjected to PCR-based genotyping for the genes such as bla (beta-lactamase) CTX-M-1, blaCTX-M-15, blaCTX-M-U, blaTEM, blaPER and SHV. Among the total 228 samples analyzed, 136 (60%) had no growth. Ninety-two (40 %) samples showed growth of E. coli and K. pneumoniae. Among the isolates that showed growth, 39 (42%) were sensitive, and the remaining 53 (57%) were resistant to third-generation cephalospor-ins. Among the isolates showed resistance, 22 (42%) were ESBL positive and 31 (58%) were ESBL negative. Among the positive ESBL, nine E. coli strains (60%) were positive for CTX-M-15 and CTX-M-1. CTX-M-15 and CTX-M-U were present in six (85%) K. pneumoniae with ESBL +. E. coli and K. pneumoniae isolated from specimens of children below ten years of age showed 41-42% ESBL producers. Prevalent ESBL-producing genes in E. coli were CTX-M-15 and CTX-M-1. CTX-M-15 and CTX-M-U were prevalent in ESBL-producing K. pneumoniae.

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来源期刊

Anti-Infective Agents Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： Anti-Infective Agents publishes original research articles, full-length/mini reviews, drug clinical trial studies and guest edited issues on all the latest and outstanding developments on the medicinal chemistry, biology, pharmacology and use of anti-infective and anti-parasitic agents. The scope of the journal covers all pre-clinical and clinical research on antimicrobials, antibacterials, antiviral, antifungal, and antiparasitic agents. Anti-Infective Agents is an essential journal for all infectious disease researchers in industry, academia and the health services.